摘要
目的调查Investigator HDplex试剂盒中所包含的12个常染色体STR基因座在华东汉族人群中的遗传学数据,考察其法医学应用价值。方法应用Investigator HDplex试剂盒对华东地区484名华东汉族健康无关个体进行常染色体STR基因座分型检测,统计分析12个常染色体STR基因座的频率数据、群体遗传学参数及连锁不平衡状况。结果 12个常染色体STR基因座在本次研究的人群中均符合Hardy-Weinberg平衡,各基因座之间相互独立。个人识别率(DP)均大于0.8,在群体中的累积个人识别率(CDP)为0.999 999 999 92,在二联体的累积非父排除率为0.999 82,在三联体的累积非父排除率为0.999 998 6。结论 Investigator HDplex试剂盒中所包含的12个常染色体STR基因座在华东汉族人群中具有良好的多态性,该试剂盒可以作为法医DNA鉴定工作的良好辅助分型工具。
Objective To investigate the genetic data of 12 autosomal STR loci included in Investigator HDplex kit and to evaluate its forensic application in Han nationality of Eastern China. Methods A total of 484 unrelated healthy individuals in Han nationality of Eastern China were investigated with Investigator HDplex kit. Allele frequencies, population genetics parameters and linkage disequilibrium information of the 12 autosomal STR loci were statistically analyzed. Results No deviations from Hardy-Weinberg equilibrium were detected and all loci were independent form each other within the studied 484 unrelated healthy individuals. DP values of the 12 autosomal STR loci were all above 0.8, and CDP was 0.999 999 999 92. The cumulative probability of paternity exclusion in duo and in trio were 0.999 82 and 0.999 998 6, respectively. Conclusion Investigator HDplex kit with 12 highly polymorphic STR loci in Han nationality of Eastern China could be used effectively for forensic DNA genotyping.
出处
《法医学杂志》
CAS
CSCD
2012年第5期351-354,共4页
Journal of Forensic Medicine
基金
国家科技支撑计划项目(2012BAK16B01)
上海市法医学重点实验室基金资助项目(12DZ2271500)
