摘要
丽斗蟋Velarifictorus ornatus具有明显的翅二型现象,长翅型与短翅型雌虫的卵巢和飞行肌存在着生理权衡。本研究分别应用蒽酮比色法、硫代磷酸香草醛法、考马斯亮蓝染液对羽化后10d内两型雌虫飞行肌与卵巢内糖原、总脂及蛋白质含量进行了定量分析。结果表明:成虫羽化后10d内,两型雌虫体重无明显差异(P>0.05),但短翅型雌虫怀卵量明显多于长翅型雌虫,而人工脱翅能够促进长翅型雌虫怀卵量增加(P<0.05)。短翅型雌虫飞行肌内蛋白质、糖原及总脂含量在成虫羽化后10d内无明显变化,但长翅型雌虫飞行肌内蛋白质在成虫羽化后3d时达到最大值564.4±87.5μg/♀,糖原与总脂含量分别于羽化后第5天达到最大值85.2±21.7μg/♀和5284.7±1267.4μg/♀。然后开始下降,各实验处理天数内,长翅型雌虫飞行肌内蛋白质、糖原及总脂含量都显著多于短翅型雌虫(P<0.05)。相反,各处理天数内,短翅型雌虫卵巢内蛋白质、糖原及总脂含量则明显多于长翅型雌虫(P<0.05),同时虫龄对蛋白质、糖原及总脂在两型雌虫飞行肌与卵巢内分配也产生明显影响(P<0.05)。人工脱翅能够促进长翅型雌虫卵巢内蛋白质、糖原及总脂含量增加,同时诱导飞行肌内蛋白质、糖原及总脂含量降低,其中总脂含量在脱翅后10d时降为2394.9±1461.8μg/♀,只有最大值的一半,而与短翅型雌虫相似(P>0.05),表明总脂为丽斗蟋飞行的主要能源物质。外用保幼激素Ⅲ能够促进长翅型雌虫卵巢内蛋白质、糖原及总脂含量增加(P<0.05),但对飞行肌内三者含量无明显影响(P>0.05),外用早熟素Ⅰ对短翅型雌虫卵巢内蛋白质、糖原及总脂含量亦无明显影响(P>0.05)。上述结果表明,丽斗蟋长翅型雌虫首先将获得的资源用于发育飞行所需的飞行肌,短翅型雌虫则首先将所获得的资源用于发育繁殖所需的卵巢,但长翅型雌虫飞行肌与卵巢间的资源分配�
The wing-dimorphic cricket Velarifictorus ornatus exists physiological trade-offs between flight muscles and ovarian development.In this study,we quantitatively analyzed the contents of protein,glycogen and total lipids in flight muscles and ovaries between the long-winged and short-winged females by coomassie brilliant blue G-250,anthrone method and sulphophosphovanillin method,respectively.At 10 d after adult emergence,there was no difference in body weight（P〉0.05） but the short-winged females loaded more eggs than long-winged females（P〈0.05）.De-alation could stimulate the long-winged females to produce more eggs than the intact long-winged females（P〈0.05）.There was no variation in contents of protein,glycogen and total lipids in flight muscles of short-winged females after adult emergence but the protein content in flight muscles of long-winged females reached the maximum（564.4±87.5 μg/♀） at day 3,while the contents of glycogen and total lipids also reached the maximum（85.2±21.7 μg/♀,5 284.7±1 267.4 μg/♀,respectively） at day 5,and then the contents of protein,glycogen and total lipids decreased from day 5.The contents of protein,glycogen and total lipids in flight muscles of long-winged females were much more than those of short-winged females at 1,3,5 and 10 d after emergence（P〈0.05）,while the contents of protein,glycogen and total lipids in ovaries of short-winged females were more than those of long-winged females after adult emergence（P〈0.05）.Age had obvious effect on the allocation of protein,glycogen and total lipids between the flight muscles and ovaries（P〈0.05）.De-alation stimulated the increase in the contents of protein,glycogen and total lipids in ovaries of long-winged females,while de-alation elicited the decrease in the contents of protein,glycogen and total lipids in flight muscles of long-winged females.At 10 d after de-alation,the contents of total lipids in flight muscles decreased to 2 394.9±1 461.8 μg/♀,which was only half of the m
出处
《昆虫学报》
CAS
CSCD
北大核心
2012年第9期1037-1045,共9页
Acta Entomologica Sinica
基金
国家自然科学基金项目(31070586)
湖南省高校创新平台开放基金项目(12K068)
中南林业科技大学青年科学研究基金重点项目(QJ2010006A)
关键词
丽斗蟋
翅二型
飞行肌
卵巢
资源分配
Velarifictorus ornatus
wing-dimorphism
flight muscles
ovary
resource allocation
