摘要
目的探讨维生素B6（VB6）缺乏对人类BRCA1突变乳腺癌淋巴细胞株基因组稳定性的影响。方法以VB6血浆正常生理浓度为依据（~20 nmol/L）,设置0,6,12,24,48,96,200 nmol/L 7个浓度组,常规RPMI1640培养基为对照（VB6浓度4800 nmol/L）,培养携带BRCA1突变的乳腺癌患者淋巴细胞株（GM13705）9 d,利用细胞质阻断微核分析（CBMN）,评价VB6缺乏对该细胞株细胞活性及遗传稳定性的影响。结果在0 nmol/L VB6浓度下,细胞不能存活;6,12 nmol/L活细胞数有下降趋势,24 nmol/L时开始增长,与初次培养时无显著性差异,48 nmol/L较24 nmol/L显著上升（P〈0.05）;在96时达最大值,与200,4800 nmol/L组间无显著性差异。双核细胞微核（MNBN）频率随VB6浓度增加显著下降,48 nmol/L时降至最低（P〈0.001～0.01）;48,96,200,4800 nmol/L时4个浓度间无显著性差异。结论 VB6浓度在96 nmol/L时是受试细胞株活性最佳浓度,48 nmol/L可维持受试细胞株遗传稳定性的最佳状态。
Objective To evaluate the effects of vitamin B6（VB6） deficiency on the genome instability in human lymphoblast cell line carrying BRCA1 mutation.Method The lymphoblast cells were cultured under the midia of 7 VB6 doses（0,6,12,24,48,96,200 nmol/L） for 9 d.Common RPMI 1640 culture was set as the control（VB6 4800 nmol/L）.The number of viable cells and genome instability index was evaluated by cytokinesis-block micronucleus assay（CBMN）.Results All cells died in 0 nmol/L,and the viable cells decreased in 6 and 12 nmol/L VB6.They became increased since 24 nmol/L and reached the peak at 96 nmol/L without significant difference with 200,4800 nmol/L groups.The frequency of micronucleated binucleated cell（MNBN） were remarkably reduced in VB6 48 nmol/L group,without significant differences with 96,200,4800 nmol/L VB6 groups.Conclusions A significant minimization effect of MNBN was found at 96 nmol/L VB6.So 96 nmol/L VB6 is the optimal concentration for cell line viable and 48 nmol/L VB6 for stabilizing the genome of the test cell line.This preliminary investigation suggests that VB6 is needed to protect genomic stability of human lymphoblant cell line.
出处
《营养学报》
CAS
CSCD
北大核心
2012年第5期441-444,共4页
Acta Nutrimenta Sinica
基金
云南省应用基础研究面上项目(No.2008CD108)
关键词
维生素B6
淋巴细胞株
胞质阻断微核分析
双核细胞微核
基因组稳定性
vitamine B6
human lymphoblast cell line
cytokinesis-block micronucleus assay
micronucleated binucleated cell
genome stability
