摘要
目的:建立以高效液相色谱法测定复方南板蓝根颗粒中靛蓝和靛玉红含量的方法。方法:色谱柱为Agilent C18(4.60 mm×150 mm,5μm),流动相甲醇-2%磷酸(梯度洗脱);流速0.9 mL.min-1,检测波长289 nm,柱温35℃,进样量20μL。结果:靛蓝、靛玉红分别在0.030 4～0.608μg(r=0.999 3)和0.0993～1.986(g(r=0.999 6)与峰面积积分值线性关系良好,平均加样回收率靛蓝为98.1%(RSD 2.31%)、靛玉红为101.4%(RSD 2.61%)。结论:该方法灵敏度高、简便、准确,可用于复方南板蓝根颗粒的质量控制。
Objective： To determine indigo blue and indirubin content in Compound South Baphicacanthus Granules by HPLC. Method： Samples were separated by Angilent Cls column （4.60 mm x 150 mm, 5 μm）, the mobile phase consisted of methonal-2% phosphate （by gradient elution） with flow rate at 0.9 mL·min^（-1）, UV detection wavelength was set at 289 nm, column temperature at 35 ℃ , and sample size was 20 p,L. Result： The linear range of indigo blue was 0. 030 4-0.0. 608 Ixg （r =0. 999 3） , and indirubin was 0. 099 3- 1. 986 tLg （ r = 0. 999 6）. The average recovery of indigo blue was 98.1% （ RSD 2.31% ） , indirubin was 101.4% （RSD 2.61% ）. Conclusion： The method is sensitive, simple, accurate, and can be used for the quality control of Compound South Baphieacanthus Granules.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第21期128-130,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
HPLC
复方南板蓝根颗粒
靛蓝
靛玉红
含量
HPLC
Compound South Baphicacanthus Granules
indigo bluer indirubin
assaying
