摘要
采用20%的聚乙二醇对金荞麦幼苗进行干旱胁迫处理,并通过添加不同体积质量分数的外源5-氨基乙酰丙酸(ALA)及一氧化氮供体硝普钠(SNP),研究外源ALA和外源NO处理对干旱胁迫下金荞麦幼苗叶片的叶绿素质量分数、可溶性糖质量分数、丙二醛(MDA)质量分数以及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性等生理指标的影响.结果显示PEG-6000(20%)胁迫下的金荞麦幼苗生长受到显著抑制,但是经过不同体积质量分数的ALA或SNP处理后,均提高了幼苗可溶性蛋白的质量分数,降低了叶片的MDA质量分数和可溶性糖质量分数,显著地提高了SOD,POD和CAT 3种酶的活性和叶绿素a、叶绿素b、总叶绿素、叶绿素a/b的水平.50mg/L的ALA处理后SOD的活性达到最大值(0.039U/(mg·min));75mg/L的ALA处理后POD的活性达到最大值(2.359U/(mg·min));0.10mmol/L的SNP处理后CAT的活性达到最大值(0.052U/(mg·min)).然而,75mg/L的ALA比50mg/L的ALA处理后,金荞麦幼苗叶片的MDA质量分数少.因此,适宜体积质量分数的ALA和SNP能够有效地减缓干旱胁迫对金荞麦幼苗产生的伤害,提高幼苗的抗干旱能力.
20% of polyethylene glycol（PEG） has been used on F.dibotrys seedling roots for drought-stricken dispose,and,by adding different concentrations of exogenous 5-Aminolevulinic acid（ALA） and nitric oxide donor sodium nitroprusside（SNP）,a research has been conducted to get physiological characteristic effects of exogenous ALA and NO on F.dibotrys seedlings under drought-stricken conditions such as the content of malondialdehyde（MDA）,soluble sugar,soluble protein,chlorophyll and the activity of superoxide dismutase（SOD）,peroxidase enzyme（POD） and catalase（CAT） have also been measured under PEG-6000（20%） stress.The result shows that F.dibotrys seedling growth is significantly inhibited,but after different concentrations of ALA or SNP treatment,the processing of improved the content of soluble protein of seedlings,decreased the of content MDA and soluble sugar,significantly improved the activity of SOD,POD and CAT and the content of chlorophyll a,chlorophyll b,total chlorophyll,chlorophyll a/b.SOD activity reached the maximum（0.039 U/（mg·min）） with the concentration of 50 mg/L ALA,POD activity reached the maximum（2.359 U/（mg·min）） with the concentration of 75mg/L ALA,CAT activity reached the maximum（0.052 U/（mg·min）） with the concentration of 0.10 mmol/L SNP.However,75 mg/L of ALA than 50 mg/L of ALA treatment F.dibotrys seedlings less MDA content.So we can draw proper density of ALA and SNP effectively mitigate drought-stricken conditions on the damage produced F.dibotrys seedlings,improve the payment ability of F.dibotrys seedlings of drought-resistant.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第10期52-58,共7页
Journal of Southwest China Normal University(Natural Science Edition)
基金
国家自然科学基金资助项目(30070080)
国家林业公益科研专项基金资助项目(201004064)
关键词
金荞麦
ALA
NO
干旱胁迫
生理特性
Fagopyrum dibotrys（D.Don） Hara
5-Aminolevulinic acid
nitric oxide
drought-stricken conditions
physiological characteristics
