摘要
目的优选通脉养心方的提取工艺。方法使用氯仿诱发小鼠室颤模型和大鼠离体胸主动脉血管环试验结果结合甘草酸含量作为指标,通过正交试验优化通脉养心方提取工艺,并进行验证。结果确定最优提取工艺为60%乙醇提取2次,第一次8倍量水提取1.5 h,第二次6倍量水提取1 h。结论工艺优化后的通脉养心方具有良好药理活性,呈现量–效关系。
Objective To optimize extraction technology of Tongmai Yangxin prescription.Methods Animal arrhythmia on mice by chloroform and isolated thoracic aortic ring of rats to observe effects of pharmacological activity and contents of glycyrrhizic acid were used to optimization and verify the extraction process of Tongmai Yangxin prescription.Results Optimum extraction conditions were as follows： heating refluxing extracted 2 times with the amount of 60% ethanol for 8 times 1.5 hours and 6 times 1 hour.Conclusion Tongmai Yangxin prescription in the optimized extraction technology with significant pharmacological activity,showing the dose-effect relationship.
出处
《药物评价研究》
CAS
2012年第4期265-269,共5页
Drug Evaluation Research
关键词
通脉养心方
提取工艺
心律失常
胸主动脉环
Tongmai Yangxin Prescription； extraction technology； arrhythmia； thoracic aortic ring
