摘要
生物受激发光探测是一种感知水中目标的新技术、新手段。选取多边膝沟藻、夜光虫和月芽形梨甲藻3种典型海洋受激发光生物体为对象,采用光谱仪、光子计数器、库埃特流场旋转圆筒等装置搭建了生物受激发光实验平台,研究了不同刺激下生物体受激发光的光学特性,并在此基础上结合概率论和数理统计理论,建立了流场刺激下的生物发光的数理模型。结果表明:发光藻的发光具有较好的单色谱特性,峰值波长集中在472 nm左右,谱线的半波宽度为30～40 nm。发光藻的发光随着机体的疲劳而变弱,但发光能力随着机体的休息会慢慢恢复。同种刺激条件下,3种藻发光强度的强弱依次为月芽形梨甲藻、夜光虫、多边膝沟藻。
The detection of stimulated bioluminescence is the new technology and method of target detection in water.Three representative luminescent alga,such as lingulodinium polyedrum,pyrocystis noctiluca and pyrocystis lunula,are studied.The platform of stimulated bioluminescence is built by the use of spectrometer,photon counter,Couette flow device and so on.The optical characteristics of bioluminescence stimulated by flow are researched.Based on the experimental study,probabilistic and mathematic statistic theory,the math-physical model of stimulated bioluminescence is built.The experiment results showed that the light spectrum of luminescence alga is a single wavelength with about 472 nm of the maximum wavelength and 30～40 nm of full width at half maximum.Furthermore,the intensities of bioluminescence are different with different organisms and attenuated with the decrease of the energy of organisms,but it will be recovered after a rest.On the basis of their bioluminescence response in the same stimulated condition,these species were ranked in order of decreasing sensitivity as I（pyrocystis lunula）I（pyrocystis noctiluca）I（lingulodinium polyedrum）.
出处
《发光学报》
EI
CAS
CSCD
北大核心
2012年第10期1039-1048,共10页
Chinese Journal of Luminescence
基金
国防基金(9140A03050809JB1106)资助项目
关键词
生物受激发光
发光藻
库埃特流
数理模型
stimulated bioluminescence
luminescent alga
Couette flow
math-physical model
