摘要
本文以体外培养的宫颈癌Hela细胞系为模型,以细胞克隆形成实验及细胞周期相分布测定为指标,观察了高温、顺铂及放射线对细胞的杀伤效应。结果表明:①从细胞克隆形成实验所得存活分数(SF)证明热化或热放二联因子较热、化、放任何单一因子对细胞的杀伤效应大,且热化优于热放。热化放三联因子应用时,杀伤作用更大。②从流式细胞仪测定的细胞周期时相分布表明,Hela细胞周期时相对热化因子较敏感,剂量小时,S+G_2/M增殖期细胞增加,呈激惹反应;剂量大时,S+G_2/M增殖期细胞减少,呈抑制反应。热放组于24小时无明显改变,96小时S+G_2/M增殖期细胞呈轻度增加现象。
In this paper the killing effects of thermo-chemo-radiotherapy were observed in Hela cells. Liner accelarator was used for the X-ray sourse. Cisplatin was used as the chemo-drug. The indexes used were the clone formative test and the cell cycle phase of flow cytometey (FCM). The results demonstrated are as follow:
(1) SF (survival fraction) by thermo-radiolherapy or thermo-chemotherapy was less than that by only single one. Thermo-chemotherapy was better than thermo-radiotherapy and triple treatment was most effective.
(2) FCM showed that the cell cycle phase was sensitive to chemotherapy. When the drug dose was small, S+G_2/M multiplication cells increased and it had the stimulation reaction. When the dose was large, S+G_2/M cells decreased and it had inhibition reaction. In addition, there was no change at 24h in cell cycle phase by radio-hyperthermia and demonstrated that S+G_2/M multiplication cells increased lightly. It suggests that the chemo-drug dose should be reasonably large and radio-therapy should be repeated 24h later in clinical treatement.
出处
《军医进修学院学报》
CAS
1990年第4期307-309,共3页
Academic Journal of Pla Postgraduate Medical School
关键词
温热疗法
化学疗法
放射疗法
肿瘤
克隆形成实验
Thermotherapy Ghemotherapy Radiotherapy
Neoplasms, experimental
Clone formative test Flow cytometry
