摘要
目的自行设计合成一种以骨缺损修复为目的的神经生长因子缓释微球,并进行理化性能和体外释放实验。方法复乳法制备mPEG-PLA-NGF缓释微球,并观察其大小形态,用ELISA法测定微球包封率及载药量,用动态透析释药法测定体外释放率。结果微球表面光滑圆整,球体大小较均匀。微球粒径为(74.2±21.3)μm,粒径分布范围较窄。包封率和载药量分别为(77.3±1.8)%和[(2.13±0.24)×10-5]%,体外释放实验中,没有发现突释现象,24 h释放率为27.36%,微球在21 d后释放度达72.34%。结论 mPEG-PLA-NGF缓释微球具有良好的物理特性和缓释效果。
Objective To prepare and investigate the characteristic of biodegradable mPEG-PLA microspheres as carriers for controlled nerve growth factor（NGF） delivery,and the sustained release rate in vitro.Methods mPEG-PLA was utilized as delivery material to prepare the NGF microspheres by multiple emulsion method.The physical surface of microspheres was detected,and the content of NGF in the microspheres was evaluated by ELISA.Dialysis was used to examine the in vitro drug release.Results The microspheres were spherical in shape,and had a smooth surface.The particle size was uniform with a size of（74.2 ± 21.3） μm.Drug-loading amount was [（2.13 ± 0.24）×10^-5]% and the entrapment rate was（77.3 ±1.8） %.There was no burst release found in vitro.The release rate was 27.36% of total amount in 24 h.The accumulative sustained release rate was 72.34% in 3 weeks.Conclusion mPEG-PLA-NGF microspheres have a good physical performance and sustained release character in vitro.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2012年第20期2075-2077,共3页
Journal of Third Military Medical University
基金
国家自然科学基金(30973334
81271098)
重庆市自然科学基金(CSTC2009BB5334)~~
