摘要
目的:研究芍药甘草汤及其拆方对H2O2诱导损伤大鼠小肠上皮细胞的保护作用。方法:实验设空白对照组,H2O2模型组,白芍(Paeoniae Radix,PR)组,甘草(Licorice,L)组,白芍甘草不同比例组。药物组在药物作用24 h后,与模型组一起加入200μmol.L-1的H2O2作用2 h,空白对照组正常培养,用MTT方法检测细胞活性,Annexin V/PI双染检测细胞凋亡。RT-qPCR检测Caspase-3和Bax mRNA表达。结果:与空白对照组比较,在H2O2损伤后,模型组细胞MTT检测吸光度(A)明显降低,存活细胞明显减少,凋亡增高,Caspase-3表达明显增高,Bax mRNA无明显变化;与H2O2模型组比较,PR 200μg.mL-1,PR 100μg.mL-1,L 100μg.mL-1,L50μg.mL-1,PR 200μg.mL-1/L100μg.mL-1,PR 100μg.mL-1,100μg.mL-1,PR100μg.mL-1,50 mg.L-1组A明显增高,细胞凋亡明显减轻,Caspase-3表达明显降低。结论:白芍,甘草单用,以及白芍与甘草配伍均对H2O2诱导损伤的小肠上皮细胞有保护作用,其机制可能与抑制Caspase-3 mRNA的表达相关。
Objective： The present study was designed to observe the effect of Paeornla Licore Tang （PR/L） and its disassembled formula Shaoyao （PR）, Ganchao （L） on damage of rat IEC-6 cells induced by H202, and also to investigate the potential mechanism. Method： IEC-6 cells were cultured with blank control medium, PR/L, PR, or L of the fixed dosages previously for 24 h, and then stimulated with 200 p, mol L-1 H202 for 2 h. Cell survival was measured by MTT assay. Cell apoptosis was evaluated by using flow cytometry with Annexin V / PI staining. Expression of Caspase-3 mRNA was detected by RT-qPCR. Result： Compared with the controls, the H202 model showed decrease in survival and expression of Caspase-3 mRNA, and increase in apoptosis. Compared with the H202 model, after treatment of PR 200 p,g -mL-1 or 100 ug -mL-1 , Gancao of 100 ug mL-1 or 50 p,g mL-1 , or both PR and L for 24 h, cell damage and cell apoptosis were reduced significantly, and expression of Caspase-3 mRNA and protein was both increased. Conclusion： Shaoyao, Gancao, and Shaoyao Ganchao Tang can protect IEC-6 cells from damage by H202, which may be due to the decrease in Caspase-3 expression.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第20期259-262,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81001563)
关键词
芍药甘草汤
拆方
H2O2诱导损伤
细胞凋亡
Shaoyao Ganchao Tang
disassembled formula
H2 02 -induced damage
cell apoptosis
