摘要
[目的]本实验通过对阿胶、铁剂对缺铁性聋大鼠耳蜗SOD含量的比较,探究阿胶与铁剂在耳蜗损伤修复功能方面的机制。[方法]选听力正常Wistar大鼠40只,雌雄各半,10只喂饲普通饲料作为对照组,30只喂饲缺铁饲料建立缺铁性聋动物模型,饲养6周后通过检测DPOAE筛选出15只造模成功的大鼠,分成模型组、铁剂组和阿胶组3组,在原有饲料的基础上分别做不给药、给铁剂、给阿胶处理,3周后检测动物血清SOD,未处理组为模型组。[结果]对照组与模型组比较有显著性差异(P<0.01),阿胶组、铁剂组与模型组比较差异均有统计学意义(P<0.05),阿胶组与铁剂组比较无显著性差异(P>0.05)。[结论]缺铁能导致SOD含量的减少,阿胶、铁剂能通过改变SOD含量来改善缺铁性聋大鼠的听力情况,其作用效果与铁剂无明显差别。
[Objective] The purpose of this research is estimating the effects caused by donkey-hide gelatin,chalybeate on the cochlea of the iron deficient hearing-loss rats,and judge which is better by testing the concentration of SOD in cochlea.[Method] Select 40 Wistar rats with normal hearing,50% females to 50% males,normal diet as control（10）group,use the forage without iron to build iron deficient deafness animal model（30）,then test their DPOAE to screen the very one which is deaf after 6 weeks feeding,and 15 ones with hearing damage are selected out.Then divide them into three groups： model group,chalybeate group and donkey-hide gelatin group,and feed with no medicine,chalybeate,donkey-hide gelatin at the random forage.After 3 weeks,test serum SOD,untreated with model group.[Result] Compare model group with control group,the result has significant change（P0.01）,and compared with model group,both donkey-hide gelatin group and chalybeate group have statistically significant difference（P0.05）,but there is no obvious difference in the side comparison of the chalybeate group and donkey-hide gelatin group（P0.05）[.Conclusion]Both donkey-hide gelatin and chalybeate have the same effect in reconditioning the hearing of iron deficient hearing loss by changing the volume of SOD.
出处
《浙江中医药大学学报》
CAS
2012年第9期1048-1051,共4页
Journal of Zhejiang Chinese Medical University
关键词
缺铁性聋
耳蜗
超氧化物歧化酶
阿胶
铁剂
iron deficient hearing-loss
cochlea
SOD
donkey-hide gelatin
chalybeate
