摘要
目的:比较斑马鱼胚胎和肿瘤细胞作为药物筛选模型的优缺点。方法:采用MTT法检测顺铂、紫杉醇、阿霉素、5-氟尿嘧啶四种药物对HL-60和Hela细胞的增殖影响;同时,观察药物对斑马鱼胚胎发育的影响。结果:阿霉素、顺铂及紫杉醇作用于HL-60及Hela细胞的IC50均显著高于作用于斑马鱼胚胎的LD50;而5-FU作用于肿瘤细胞和斑马鱼胚胎的结果与其它药物相反;四种抗肿瘤药物对斑马鱼胚胎的生长发育均有致畸作用。结论:斑马鱼胚胎作为细胞毒类药物筛选模型,对于抗微管类药物较为敏感,但对于抗代谢药敏感性较肿瘤细胞差。
Objective： To investigate the differences in cytotoxic drug screening with zebrafish embryos and tumor cells. Methods： Human leukemia cells （HL-60） and cervical cancer cells （Hela） were used to evaluate the cytotoxic effect of four different antineoplastics （paclitaxel, 5-fluorouracil, doxorubicin and cisplatin） with MTT assay. And the impact of the four drugs on zebrafish embryos development was also obersseved. Results： The data demonstrated that the half inhibitory concentration （IC50） ofpaclitaxel, doxorubicin and cisplatin on cancer cells significantly exceeded the LD50 of the three drugs on zebrafish embryos. However the effect of 5-fluorouracil was opposite. And the four drugs had teratogenic effect on zebrafish embryos. Conclusion： Zebrafish and embryos as a new model for drug screening in cytotoxic drugs, is more susceptible to the inhibitor of microtubule formation and less susceptible to antimetabolites than tumor cells.
出处
《现代生物医学进展》
CAS
2012年第27期5214-5217,5287,共5页
Progress in Modern Biomedicine
基金
教育部新世纪优秀人才支持计划(NCET-10-0967)
国家自然科学基金项目(11175222)
兵团科技攻关计划(2011GG11)
石河子大学自然科学重点项目(ZRKX2009ZD01)
山东省优秀中青年科学家奖励基金(2006BS01226)
山东省自然基金项目(ZR2009BM027&Y2008B51)
关键词
斑马鱼
胚胎
肿瘤细胞
抗肿瘤药
Zebrafish
Embryos
Tumor cells
Antineoplastic drugs
