摘要
建立了采用固相萃取法(SPE)富集、HPLC法检测微囊藻毒素(MC-LR)的分析方法,采用80%的甲醇溶液按1∶50的质量体积比,在室温下磁力搅拌抽提2 h,于8 000 r/min转速下离心20 min,取出上清液,利用旋转蒸发仪脱除提取液中的甲醇,采用蛋白质等电点沉淀法进一步去除色素,最后利用SPE富集纯化MC-LR。该方法具有较好的测定准确度、精密度和回收率,且提取率高、藻毒素中杂质含量低,检测限为0.1 mg/L,适用于分析水体中的痕量MC-LR。
The enrichment and detection of microcystin-LR were performed by the solid phase ex- traction （SPE） and high performance liquid chromatography （HPLC）. The mierocystin-LR was extracted with 80% aqueous methanol （re^V） stirring for 2 h at room temperature when the fluid solid ratio was 1 ： 50. The supernatant was obtained after centrifugation （20 rain, 8 000 r/rain）. The methanol in the extract was removed by the rotary evaporator, and the color was removed by the protein isoelectrie point precipitation process. SPE was used for the microcystin-LR concentration and partial purification. The microcystin-LR was identified and quantified by HPLC. The method has good accuracy, precision, recov- ery rate, high extraction rate and low impurity content. The minimum detection limit was 0.1 mg/L. The method is suitable for analyzing the trace microcystin-LR in water.
出处
《中国给水排水》
CAS
CSCD
北大核心
2012年第20期142-145,共4页
China Water & Wastewater
基金
山西省科技基础条件平台建设项目(2009091015)
山西省软科学研究项目(2008041033-03)
