摘要
目的探讨血清肝型脂肪酸结合蛋白在大鼠肝脏缺血再灌注损伤的早期诊断价值。方法雄性Wistar大鼠随机分为3组：假手术组（A组）；缺血15min再灌注组（B组）；缺血30min再灌注组（C组）。建立肝缺血再灌注损伤模型，分别在缺血再灌注后15min、1h、3h、6h、1d、3d检测血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）水平和肝型脂肪酸结合蛋白（L-FABP）含量；免疫组织荧光法检测肝脏L—FABP的表达；观察肝组织病理学改变并对其进行suzuki’s评分。结果与A组比较，B组血清L—FABP变化：再灌注15min，血清L—FABP水平升高[（0．57±0．14）μg／L，P〈0．05]，峰值出现于再灌注3h[（1．70±0．26）肛g／L，P〈0．05]，3d基本降至正常[（O．16±0．05）μg/L，P〉0．05]；血清ALT、AST变化：再灌注15min无明显升高，6h达高峰，3d仍高于正常（P〈0．05）；肝组织L—FABP变化：再灌注15min表达下降（0．148±0．047，P〈0．05）；3h时处于谷值（0．071±0．019，P〈0．05）；3d时基本恢复正常（0．142±0．047，P〉0．05）。与B组比较，C组各时间点血清L—FABP、AST和ALT水平均明显升高（P〈0，05），肝组织L—FABP表达明显降低（P〈0．05），病理学改变明显加重。结论与传统肝功能指标（ALT、AST）比较，L—FABP是一种监测肝脏缺血再灌注损害的更为敏感的指标，与肝组织病理学变化趋势一致。
Objective To investigate the diagnostic value and significance of serum liver fatty acid-binding protein （L-FABP） in hepatic ischemia-reperfusion injury in rat. Methods Male Wistar rats were randomly divided into three groups： sham operation group （group A） ; reperfusion after 15 min of ischemia group （group B） ; reperfusion after 30 min of ischemia group （group C）. Each group was divided into 6 subgroups based on the time of reperfusion （15 min, 1 h, 3 h, 6 h, 1 d, 3 d）. The model of hepatic ischemia-reperfusion injury was established, the level of alanine aminotransferase （ALT）, aspartate aminotransferase （AST） and L-FABP were tested at each time point and the expression of L-FABP was tested by Immunohistochemical Fluorescence. The pathological changes observed in the liver and evaluated the changes by Suzuki＇ s scoring system. Restilts Compared with group A, the changes of serum L-FABP： increased after 15 min of reperfusion [ （0./57± 0. 14） μg/L, P 〈 0. 05 ], reached the peak after 3 h of reperfusion [ （ 1.70 ±0. 26） μg/L, P 〈0./05] and returned to normal at 3 d [ （0. 16 ±0. 05） μg/L, P 〉 0. 05] ; the changes of serum ALT and AST： no significam increase after 15rain of reperfusion, reach the top at 6h and the level was still higher than normal at 3 d （P 〈0. 05） ; L-FABP in liver tissue： the expres- sion was decreased after 15rain of reperfusion （0. 148 ± 0. 047, P 〈 0. 05 ）, reached to the trough at 3 h （0.071 ±0.019, P〈0.05） and returned to normal at 3 d （0. 142 ±0.047, P〉0.05）. Compared with group B, the level of serum L-FABP, AST and ALT in group C were significantly increased at each time point （P 〈 0. 05 ）, and the expression of L-FABP was significantly decreased （ P 〈 0.05 ）, the pathological changes were significantly worse. Conclusion Compared with the traditional indicator of liver function （ALT, AST）＇, L-FABP is the more sensitive indicator to monitor the hepatic ischemia-reperfusion injury, and
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第10期1940-1943,共4页
Chinese Journal of Experimental Surgery
基金
天津市卫生局重点资助项目（2010KR05）
关键词
肝脏
缺血再灌注损伤
肝型脂肪酸结合蛋白
Liver
Ischemia-Reperfusion Injury
Liver fatty acid-binding protein
