摘要
目的 :探讨门静脉血 K- ras、p5 3基因突变与大肠癌肝转移的关系。方法 :应用 PCR- SSCP和 PCR-RFL P方法 ,对大肠癌患者的癌组织、门静脉血、外周静脉血分别进行 K- ras、p5 3基因检测 ,术后随访 2 a,了解肝转移情况。结果 :30例中门静脉血 K- ras基因突变 6例 ,无突变 2 4例 ,术后 2 a内肝转移率分别为 6 6 .7% (4/6 )、16 .7% (4/2 4) ,两者差异有统计学意义 (P<0 .0 2 5 ) ;30例中门静脉血 p5 3基因突变 10例 ,无突变 2 0例 ,术后 2 a内肝转移率分别为 6 0 % (6 /10 )、10 % (2 /2 0 ) ,两者差异有统计学意义 (P<0 .0 0 1)。结论 :检测门静脉血 K- ras、p5 3基因突变对预测大肠癌肝转移 ,判断预后有重要意义。
Objective:To explore the relationship between K ras and p53 gene mutation in portal vein blood and hepatic metastasis from large intestine carcinoma.Methods: By PCR SSCP and PCR RFLP, K ras and p53 gene mutation in carcinoma tissues,protal vein blood and peripheral blood from large intestine carcinoma patients were studied,and a two year post operation follow up of hepatic metastasis was made.Results:K ras gene mutation in portal vein blood were founded in 6 of 30 cases,hepatic metastasis rates were 66.7%(4/6) and 16.7%(4/24),(P<0.025);p53 gene mutation in portal vein blood found in 10 of 30 cases,hepatic metastasis rate was 60%(6/10) and 10%(2/20)(P<0.01).Conclusion:Detection of K ras and p53 gene mutation in portal vein blood can help to predict hepatic metastasis and judge prognosis of patients with large intestine carcinoma.
出处
《肿瘤研究与临床》
CAS
2000年第3期161-163,共3页
Cancer Research and Clinic
关键词
大肠癌
门静脉
K-RAS基因
P53基因
肝转移
Large intestine carcinoma
Portal vein
K ras gene
p53 gene
Hepatic metastasis
