摘要
目的:研究菊明提取物中降压活性物质黄酮类成分的质量控制标准。方法:以蒙花苷和野菊花对照药材为对照品,采用薄层色谱法(TLC)鉴别方中野菊花。以芦丁为对照品,采用亚硝酸钠-硝酸铝-氢氧化钠显色法测定总黄酮含量;采用高效液相色谱法(HPLC)测定蒙花苷含量,Discovery C18(250mm×4.6mm,5μm),流动相为甲醇-2%醋酸(52:48),检测波长为334nm。结果:薄层斑点清晰,专属性强。芦丁对照品线性范围在13.37-66.84μg/mL,回归方程为Y=0.0129X-0.0116,r=0.9999;平均回收率为98.28%,RSD为2.05%。蒙花苷对照品线性范围在21.90-1 752.00μg/mL,回归方程为Y=92845X+401863,r=0.9999;平均回收率为101.83%,RSD为1.10%。结论:建立的薄层鉴别和含量测定方法简便可行、结果准确,可用于菊明提取物中黄酮类成分的质量控制。
Objective： To study the quality control standard of flavonoids from Juming extracts. Methods： TLC was adopted to distinguish the Chrysanthemi Indici Flos with rutin and the medicinal material as reference substance. The content of total flavonoids was determined by NaNO2-Al（NO3）3-NaOH with rutin as reference substance. Linarin was determined by HPLC with C18 （250mm×4.6mm, 51μm）, methanol-2%HAc （52：48） as mobile phase and 334nm as detection wavelength. Results： The spots in TLC were clear and distinctive. Linear relation of rutin was 13.37-66.84μg/mL and the regression equation was Y=0.0129X-0.0116, r=0.9999. The average recovery was 98.28% with RSD 2.05%. Linear range of linarin was 21.9-1 752μg/mL and the regression equation was Y=92845X＋401863, r=0.9999. The average recovery rate was 101.83% with RSD 1.10%. Conclusion： The methods were simple and accurate. It can be used for the quality control standard of Juming extracts.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2012年第10期2705-2707,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
浙江中医药大学科技创新团队[No.浙中大发(2011)143]~~
