摘要
采用CTAB法、改良SDS法、高盐低pH法提取了剑叶龙血树叶DNA,并通过紫外分光光度计、琼脂糖凝胶电泳检测了不同方法的提取效果.结果表明:CTAB法所提取的DNA浓度与纯度均高于其它2种方法,泳带分布集中,无拖尾现象,A260/A280=1.81,DNA浓度为735 ng/μL,能满足RAPD、SSR/ISSR扩增的要求,是提取龙血树叶片DNA的最佳方法.
The genomic DNA from the leaves of Dracaena cochinchinenis were extraction by CTAB, SDS, high salt low pH method and detected by ultraviolet spectrophotometer and agarose gel electrophoresis. The results indicated that the concentration and purity of DNA extracted with CTAB were higher than the other two methods. Its DNA bands were centralized without tailing and A260/A280 = 1.81. The concentration of DNA was 735 ng/uL which could meet the requirements of RAPD, SSR/ISSR amplification. So CTAB method was the most effective method to extract the genomic DNA from Dracaena cochinchinenis leaves.
出处
《中南民族大学学报（自然科学版）》
CAS
2012年第3期59-62,共4页
Journal of South-Central University for Nationalities：Natural Science Edition
基金
中央高校基本科研业务费专项资金资助项目(CZY12016)
中南民族大学自然科学基金资助项目(YZQ10009)
