摘要
目的探讨HBSAg阳性原发性肝癌(HCC)患者血清蛋白电泳谱的特点,并揭示相关蛋白质的检测意义。方法采用美国Helena REP3蛋白电泳仪对乙型肝炎病毒表面抗原(HBSAg)阳性原发性肝癌患者及其他HBV感染性肝病患者的血清蛋白电泳进行分析,同时检测血清清蛋白(ALB)、α1抗胰蛋白酶(AAT)、甲胎蛋白(AFP)、触珠蛋白(Hp)、铜蓝蛋白(Cp)、转铁蛋白(TRF)、补体C3(C3)、免疫球蛋白G(IgG)、免疫球蛋白A(IgA)、免疫球蛋白M(IgM)、C反应蛋白(CRP),并以健康成年人为对照组,对检测结果进行统计学分析。结果 HCC患者清蛋白百分比最低,与正常对照组、慢乙肝组比较差异均有统计学意义(P<0.05);而α1-球蛋白百分比最高,与正常对照组、乙肝肝硬化组比较差异均有统计学意义(P<0.05),α2-球蛋白百分比也显著高于其他疾病组。HCC患者血清AAT、AFP、Cp、CRP含量显著高于其他组(P<0.05);Hp、C3除了比正常对照组稍低外,也显著高于其他疾病组(P<0.05)。结论 HBSAg阳性原发性肝癌患者血清蛋白电泳谱的改变主要表现在清蛋白降低,而α(包括α1和α2)球蛋白相对其他疾病组升高,α球蛋白的升高与正向急性时相蛋白及AFP的增加有关。血清蛋白电泳结合相关特定蛋白的检测对疾病的诊断及病情发展变化的评估具有重要意义。
tocellular carcinoma and explore the clinical implication of related serum proteins. Methods The Helena REP3 agarose gel electrophore- sis technique was used for serum protein electrophoresis in patients with hepatitis B virus - associated hepatocellular carcinoma,other liver disease patients with chronichepatitis B virus infection and healthy adults. Meantime, serum albumin（ALB） ,α1 -antitrypsin（AAT） , al- phafetoprotein （ AFP）, haptoglobin （ Hp）, Ceruloplasmin （ Cp ）, transferrin （ TRF）, complement C3 （ C3 ）, immunoglobulin G （ IgG）, immu- noglobulin A （IgA） ,immunoglobulin M （IgM） and C -reactive protein（CRP） were detected. Then a statistical analysis was made on these results. Results In all groups,the percent of albumin in patients with hepatitis B virus - associated hepatocellular carcinoma was lowest, and the difference compared with healthy aduhs and patients with chronic hepatitis B had statistical significant（ P 〈 0. 05 ）. The percent of α1-globin was highest, and the difference compared with healthy adults and patients with posthepatitic cirrhosis had statistical signifi- cance（ P 〈 0.05 ）. The percent of α2 - globin was higher than other patients. And the level of serum AAT, AFP, Cp and CRP was signifi- cantly higher than that in other groups （P 〈 0.05 ）. The level of serum Hp and C3 was significantly higher than that in other patients（just a little lower than that in healthy adults）. Conclusion The changes of serum protein electrophoresis in patients with hepatitis B virus - associated hepatocellular carcinoma were mainly presented with the decreased level in albumin and increased percent of α1 and α2 - glo- bins. Increased percent of α - globins markedly related with increased positive acute phase proteins and AFP. Serum protein electrophoresis combined with detection of specific proteins was important for the diagnosis of disease and the assessment of disease progression.
出处
《医学研究杂志》
2012年第9期133-136,共4页
Journal of Medical Research
基金
吴阶平医学基金会肝病实验诊断研究基金资助项目(LDWMF-SY-2011C004)
宁波市自然科学基金资助项目(2010A610056)
关键词
乙型肝炎病毒
原发性肝癌
血清蛋白
电泳
急性时相蛋白
Hepatitis B virus
Hepatocellular carcinoma
Serum proteins
Electrophoresis
Acute phase proteins
