摘要
Dear Editor, As an essential element for pollen tube tip growth, actJn cytoskeleton has been a focus for decades. Recently, both rapid-freeze and room-temperature fixation have revealed the presence of a subapical cortical F-actin （actin fringe） as a consistent feature of lily pollen tubes （Lovy-Wheeler et al., 2005）. The actin fringe consists of a palisade of short longitudinally oriented actin cables that start about 1-5~m behind the extreme apex and extend basally for an additional 5-10pm （Lovy-Wheeler et al., 2005）. MeanwhJJe, observations in live cells using Lifeact-mEGFP, an actin probe consisting of the first 17 amino acids from the budding yeast ABP-140 fused to GFP, which does not affect the dynamics of actin, compellingly support the presence of a cortical actin fringe in lilv pollen tubes ~Vidali et al.. 2009~.
Dear Editor, As an essential element for pollen tube tip growth, actJn cytoskeleton has been a focus for decades. Recently, both rapid-freeze and room-temperature fixation have revealed the presence of a subapical cortical F-actin （actin fringe） as a consistent feature of lily pollen tubes （Lovy-Wheeler et al., 2005）. The actin fringe consists of a palisade of short longitudinally oriented actin cables that start about 1-5~m behind the extreme apex and extend basally for an additional 5-10pm （Lovy-Wheeler et al., 2005）. MeanwhJJe, observations in live cells using Lifeact-mEGFP, an actin probe consisting of the first 17 amino acids from the budding yeast ABP-140 fused to GFP, which does not affect the dynamics of actin, compellingly support the presence of a cortical actin fringe in lilv pollen tubes ~Vidali et al.. 2009~.
