摘要
目的分析和鉴定白芥子中脂肪酸及其他脂溶性成分。方法将白芥子粉碎后过40目筛,用石油醚(60～90℃)回流提取,提取液经浓缩后进行甲酯化,并由气相色谱-质谱联用技术(GC-MS)对其脂肪酸及其他脂溶性成分进行分析和鉴定。结果从白芥子石油醚提取物中检测出23种物质,鉴定出其中的18种脂肪酸和5种非脂肪酸成分。主要脂肪酸成分为:芥酸(45.93%)、油酸(23.66%)、亚油酸(9.72%)和顺-11-二十碳烯酸(4.81%),以及少见的脂肪酸2-己烷基环丙烷癸酸和2-己烷基环丙烷辛酸。结论白芥子中不饱和脂肪酸为其主要成分,主要为芥酸、油酸、亚油酸和顺-11-二十碳烯酸的含量较高。
Objective To analyze and identify fatty acids and other liposoluble components in seeds of Sinapis alba.Methods Seeds of Sinapis alba were shattered into powder,sifted through 40 mesh fineness,extracted by petroleum ether heating reflux extraction（60~90 ℃）,concentrated and performed methyl esterification.Finally fatty acids and other liposoluble components were determined and identified by using GC-MS.Results 23 kinds of substances were detected in petroleum ether extract of seeds of Sinapis alba.18 kinds of fatty acid and 5 kinds of non fatty acid compositions were identified.The major fatty acids components were erucic acid（45.93%）,oleic acid（23.66%）,linoleic acid（9.72%）,cis-11-eicosapentaenoic acid（4.81%） as well as the rare fatty acids of 2-hexyl-cyclopropanedecanoic acid and 2-hexyl-cyclopropaneoctanoic acid.Conclusion Unsaturated fatty acids are the major components in seeds of Sinapis alba.Among them,erucic acid,oleic acid,linoleic acid and cis-11-eicosapentaenoic acid have the higher content.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第26期2749-2750,共2页
Chongqing medicine
关键词
主成分分析
脂肪酸类
色谱法
气相
质谱法
白芥
principal component analysis
fatty acids
chromatography,gas
mass spectrometry
Sinapis alba
