摘要
目的探讨脑心通对大鼠脑缺血后神经细胞凋亡的保护作用。方法应用线栓法制备大鼠脑缺血再灌注模型。将大鼠分为假手术组、模型组、低剂量组和高剂量组,各组分别于术前3d和术后给予不同剂量药物灌胃,术后24、48、72、96h,取脑组织进行HE染色、TTC染色、测定Caspase-3表达的动态变化。结果脑心通低、高剂量组均能减少大鼠脑梗死体积(P<0.05),减少Caspase-3阳性细胞表达。结论脑心通胶囊对大鼠局灶性脑缺血再灌注后神经细胞凋亡有保护作用。
Objective To investigate the protective effects of Naoxintong Capsules（NCs） against the cerebral damage in the rat ex- perienced cerebral reperfusion. Methods The rat model ofcerebral reperfusion with thread embolism was made. Ratsults Randomly divided into four groups： sham operation groups model group, low does treatment group and high does treat- ment group. Each group was given difference does medicine three days before the operation and after the operation. At the time of 24h,48h,72h and 96h after opration, the cerebral infarct size, the expression of Caspase-3 were observed , HE staining, TIC staining and Immunohistochemistry technique. Results NCs low does and high does all could reduce the cerebral infarct size（P〈0.05）; reduce the expression of Caspase-3（P〈0.05） in the rats. Conclusion The results sug- gest that NCs posses protective effects against the cerebral damage caused by cerebral reperfusion.
出处
《中外医疗》
2012年第21期27-27,29,共2页
China & Foreign Medical Treatment
