摘要
【目的】克隆盐芥STZ转录因子基因,对其进行序列分析,为进一步研究ThSTZ转录因子在逆境应答中的作用奠定基础。【方法】利用GenBank数据库中盐芥STZ转录因子的EST序列设计特异引物,通过3′RACE技术克隆ThSTZ基因的全长序列,应用生物信息学手段对该基因编码蛋白的结构与功能、亚细胞定位等进行预测,并对其编码氨基酸序列的同源性和系统进化进行分析。【结果】盐芥STZ转录因子基因全长717bp,编码238个氨基酸。其编码蛋白含有2个C2H2型锌指结构域,位于细胞核内,属于C2H2家族转录因子;多重序列比对和系统进化分析表明,盐芥STZ转录因子与拟南芥属植物同源蛋白的相似性最高。【结论】获得了ThSTZ转录因子基因的全长序列,为进一步探讨ThSTZ转录因子在逆境应答中的功能奠定了基础。
【Objective】 In the study,the coding region of ThSTZ（Thellungiella halophila STZ） was isolated and analyzed by using bioinformatics tools,which will provide a theoretical basis for further functional analysis of ThSTZ transcription factor in stress response.【Method】 A pair of specific primer was designed based on EST sequences of ThSTZ from GenBank database.The coding region of ThSTZ gene was isolated by 3′RACE technique.The structure,function and subcellular location of ThSTZ protein were analyzed by bioinformatics software.System evolution tree of ThSTZ gene was constructed.【Result】 The coding sequence of ThSTZ gene is 717 bp in length,and encodes a 238 amino acid sequence which includes two C2H2 zinc-finger domain and located in nucleus.ThSTZ belongs to the C2H2 transcription factor family.Multiple sequence alignment and system evolution analysis of ThSTZ protein revealed that ThSTZ transcription factor has highest identity with homologous protein in Arabidopsis.【Conclusion】 A full length of the STZ transcription factor gene in T.halophila was isolated and analyzed.This study will provide a further functional analysis of ThSTZ transcription factor in stress response.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2012年第9期215-220,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
安徽省教育厅自然科学研究项目(KJ2012A215)
国家自然科学基金项目(31070361)
教育部科学技术研究重点项目(210266)
中央高校基本科研业务费专项(1112KYQN31
0910KYZY43)
国家985工程项目(MUC98507-08)
高等学校学科创新引智计划项目(B08044)
国家民委科研项目(10ZY01)
关键词
盐芥
C2H2型锌指蛋白
转录因子STZ
Thellungiella halophila
C2H2 zinc-finger protein
transcription factor STZ
