摘要
目的:探讨FOXM1对涎腺腺样囊性癌细胞增殖的影响。方法:以高转移腺样囊性癌细胞系(SACC-M)为对象,构建FOXM1 siRNA干扰片段,瞬转SACC-M细胞,蛋白质免疫印迹法检测干扰效率;流式细胞仪分析FOXM1干扰后SACC-M细胞周期的改变,甲基噻唑基四唑比色法检测干扰后SACC-M细胞的增殖,蛋白质免疫印迹法检测干扰后细胞周期蛋白CyclinB、CDC2的变化,划痕实验检测干扰前后细胞的迁移能力。结果:干扰后SACC-M细胞FOXM1表达明显下调,细胞增殖受到抑制,细胞周期S期比例明显下降,CyclinB表达明显下降,但细胞的迁移能力没有明显的变化。结论:FOXM1可调节涎腺腺样囊性癌细胞的增殖,但对其迁移能力没有明显作用。
Objective：To study the effect of FOXM1 gene on proliferation of salivary adenoid cystic carcinoma cells. Methods：The highly metastatic human salivary adenoid cystic carcinoma cell line （SAC＇C-M） was transient transfected by constructing FOXM1 siRNA interference clips. The interference efficiency was examined by Western＇blotting. Cell cycle changes were determined with flow cytome- try. Cell proliferation was evaluated by MTI＇. The protein expression of CyclinB, CDC2 was examined by Western blotting. Cell migra- tion was examined by wound-healing assay. Results： FOXM1 siRNA transfection specifically down-regulated the FOXM1 protein in SACC-M cells. Proliferation of SACC-M ceils was suppressed, and the percentage of S stage cells was significantly reduced. But cells migrating did not show a clear change. Conclusions：FOXM1 could regulate cell proliferation of SACC-M, but had no obvious effects on cell migration.
出处
《口腔生物医学》
2012年第3期120-123,共4页
Oral Biomedicine
基金
江苏高校优势学科建设工程资助项目
关键词
腺样囊性癌
FOXM1
增殖
RNA干扰
Adenoid cystic carcinoma
FOXM1
Proliferation
RNA interference
