摘要
目的研究不同强度的磁性附着体模拟静磁场对成骨细胞转化生长因子β1(TGF-β1)基因表达的影响。方法利用细胞静磁场加载装置,对体外培养的SD大鼠成骨细胞分别进行12.5、125和250mT的静磁场加载,连续加载0.5、1、3、5和7d,采用反转录聚合酶链反应(RT-PCR)技术检测成骨细胞的TGF-β1mRNA表达的。结果 12.5mT磁场加载组在加载0.5、1和3d,细胞的TGF-β1mRNA的表达比对照组相应时段明显增强(P<0.05)。125和250mT磁场组加载静磁场0.5、1、3、5和7d后,细胞的TGF-β1mRNA的表达升高更加明显,与对照组和12.5mT磁场加载组细胞相比差异有统计学意义(P<0.05)。静磁场对细胞的TGF-β1mRNA表达的影响有一定的剂量-效应关系。结论在本试验条件下,磁性附着体模拟静磁场可以抑制TGF-β1mRNA基因表达的下降,促进成骨细胞的分化。
Objective To explore the effects of static magnetic fields （SMF） of dental magnetic attachment system with different intensities on the expression of TGF-β1 mRNA of rat osteoblasts. Methods The cultured rat osteoblasts were exposed continuously to 12.5 roT, 125 mT, and 250 mT SMF for 0.5, l, 3, 5, and 7 days, respectively. While, controls were also set up free of SMF. Realtime quantitative PCR was employed to detect the gene expression of cellular TGF-β1 mRNA and the statistical analysis was performed. Results Osteoblasts exposed to 12.5 mT SMF for 0.5, 1, and 3 days exhibited an increase on cellular TGF-β1 mRNA expression, as compared to those of the control group （P 〈 0.05）. Moreover, after 0.5, 1, 3, 5, and 7 days SMF exposure the cellular TGF-β1 mRNA expression in 125 mT and 250 mT groups were significantly higher than those of the control group and the 12.5 mT group （P 〈 0.05）. It showed a dose-effect relationship between the magnetic intensity and the expression of cellular TGF-β1 mRNA. Conclusions Under this experimental condition, the static magnetic fields of magnetic attachments can stimulate the expression of cellular TGF-β1 mRNA in rat osteoblasts, as to enchance the differentiation of osteoblasts.
出处
《中华口腔医学研究杂志（电子版）》
CAS
2012年第4期23-26,共4页
Chinese Journal of Stomatological Research(Electronic Edition)
基金
广东省自然科学基金博士启动项目(9451008901002644)
关键词
磁性附着体
静磁场
成骨细胞
转化生长因子
Magnetic attachments
Static magnetic fields
Osteoblasts
Transforming growthfactor
