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IL-24对瘢痕疙瘩成纤维细胞增殖、细胞周期和缺氧诱导因子-1α表达的影响 被引量:1

Effect of IL-24 on proliferation,cell cycle and HIF-1α expression of keloid fibroblasts
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摘要 目的观察IL-24对瘢痕疙瘩成纤维细胞(KF)的抑制效应及缺氧诱导因子-1α(HIF-1α)表达的影响。方法建立KF组、携带绿色荧光蛋白表达质粒的KF组(pEGFP-KF组)、携带转染IL-24基因表达质粒的KF组(IL-24-pEGFP-KF组),用噻唑蓝法及流式细胞术检测KF增殖、细胞周期变化。建立KF、pEGFP-KF、IL-24-pEGFP-KF细胞株裸鼠移植瘤模型,免疫组织化学检测KF中HIF-1α表达。结果 IL-24-pEGFP-KF组较pEGFP-KF组、KF组到达平台期延迟,IL-24明显抑制了细胞增殖(P<0.05)。IL-24-pEGFP-KF组的G0/G1期比例明显高于pEGFP-KF组、KF组,而S、G2/M期比例明显降低(P<0.05)。IL-24-pEGFP-KF组的HIF-1α表达明显低于pEGFP-KF组和KF组。结论 IL-24可抑制KF增殖和HIF-1α表达,阻止细胞周期于G0/G1期。 Objective To investigate the inhibitory effect and hypoxia inducible factor 1 α (HIF-1α ) expression of IL-24 on keloid fibroblasts (KF). Methods KF proliferation and eel1 cycle in KF group, pEGFP-KF group (carrying green fluorescent protein plasmid) and IL-24-pEGFP-KF group (carrying green fuorescent protein and IL-24 plasmid) were determined by MTT and flow cytometry, respectively. HIF-1 a expression was detected by immunohistochemical technique in the xenograit tumor models ofKF, pEGFP-KF and IL-24-pEGFP-KF cell lines in nude mice. Results The arrival of the plateau phase in cell growth curves was slower in IL-24-pEGFP-KF group than that in pEGFP-KF and KF groups, indicating the inhibitory role of IL-24 in KF proliferation (P〈0.05). Compared with pEGFP-KF and KF groups, the proportion of G0/G1 phase increased, but that of S and G2/M phase decreased in IL-24-pEGFP-KF (P〈0.05). Expression ofHIF-1 a protein was lower in IL-24- pEGFP-KF group than that in pEGFP-KF and KF groups. Conclusion IL-24 can inhibit KF proliferation and HIF-1 αexpression and arrest the cell cycle in G0/G1 phase.
出处 《广东医学院学报》 2012年第3期237-239,242,共4页 Journal of Guangdong Medical College
基金 广东省自然科学基金(No.06028957)
关键词 白介素24 缺氧诱导因子-1Α 瘢痕疙瘩 intcrlculdn-24 hypoxia inducible facot 1 α kcloid
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