摘要
采用16%单宁酸作为2 000Da以上的高分子蛋白的沉淀剂,利用自动定氮仪和氨基酸自动分析仪分别检测滤液中蛋白质和游离氨基酸的含量,并计算出寡肽的含量。结果显示,测得1 500Da的低分子量蛋白的回收率为90%～95%,相对标准偏差RSD为1.6%。
16% tannic acid was selected as precipitation agent for the polymer protein over 2000Da in Fermented fishmeal. The contents of proteins and free amino acids in the filtrate of fermented fishmeal were determined by the fully automatic azotometer and automatic amino acid analyzer, and then calculated the contents of oligopeptides. The results showed that the recovery rates of low molecular weight proteins of 1500Da were 90 %-95 % and the relative standard deviation was 1.6 %.
出处
《福建农业学报》
CAS
2012年第7期747-749,共3页
Fujian Journal of Agricultural Sciences
关键词
发酵鱼粉
蛋白质
游离氨基酸
寡肽
单宁
仪器测定
Fermented fishmeal
free amino aeids
Oligopeptides
Tannin
determination
