摘要
采用气相色谱一质谱联用法对十味香鹿胶囊的挥发油成分进行分析。采用2010年版《中国药典》挥发油测定法对十味香鹿胶囊挥发油进行提取，以毛细管柱进行分析，面积归一化法测定百分含量，并对其化学成分进行鉴定。色谱柱为Agilent19091M-436型弹性石英毛细管色谱柱（O．25mm×30m，0．25μm）；程序升温：60℃，以100C／min升至280℃，保持12min。进样口温度为280℃。质谱条件：EI离子源，电子能量70eV，离子源温度230℃；扫描范围20—500amu。共检出33个化合物，鉴定出其中的22个化学成分，相对含量超过5％的成分有棕榈酸（54．107％）、顺-9-十八烯酸甲酯（8．092％）、反-9-十八碳烯酸（7．285％）、（Z，Z）-9，12-十八碳二烯酸（6．026％）、9-反十六碳烯酸甲酯（5．456％），同时鉴定出具有镇痛作用的丁香酚甲醚（0．172％）。十味香鹿胶囊中挥发油成分主要包含棕榈酸、烯酸以及烯酸甲酯类成分等。
To analyze the volatile oil components from Shiwei Xianglu Capsule by GC - MS. The volatile oil components were extracted and iso-lated with steam distillation method according to ChP（2010）. The chemical compositions were separated by GC capillary column chromatogra-phy,and the relative contents were analyzed by GC-MS.The GC - MS analysis with an Agilent 19091M- 436 capillary column （O.25mm×30m,0.25μm） ;The inlet temperature was maintained at 280 .The column oven was begun at 60 ,then programmed from 60 to 280℃ at 10/min, held for 12 min. Mass spectrometer conditions were： ionization mode, EI; electron energy 70eV;Ion source temperature 230. The mass selective detector was operated in the TIC mode, mass scan range, 20 - 500 amu. 33 compounds were detected and 22 compounds of them were identified. And the compounds that the relative contents were more than 5 % were Methyl hexadec - 9 - enoate（5.456% ）, palmitic acid （54.107 % ）, （ Z ） - 9 - Octadecenoic acid methyl ester （ 8.092 % ）, （ Z, Z） - 9.12 - Octadecadienoic acid （ 6.026 % ）, （ E ） - 9 - Octade - cenoic acid（7.285% ）.The main components in the oil were palrnfi＇tic acid, olefine acid,ene acid methyl ester.
出处
《特产研究》
2012年第3期54-56,共3页
Special Wild Economic Animal and Plant Research
基金
吉林省科技厅中药现代化项目(20050932)
