摘要
目的:探讨五倍子提取物鞣花酸抗鼻咽癌细胞的生物学活性及其分子机制。方法:体外培养鼻咽癌5-8F细胞,用2,4,6μg/mL鞣花酸处理48 h后,采用M实验分析细胞的增殖;Hoechst33258染色分析细胞凋亡;流式细胞仪分析细胞周期;Western印迹检测蛋白表达。结果:鞣花酸对5-8F细胞增殖有抑制作用,2,4,6μg/mL抑制率分别为(29.35±4.95)%,(53.32±4.44)%和(61.75±6.93)%,与对照组比较,差异有统计学意义(P<0.01);2,4,6μg/mL鞣花酸处理组S期细胞百分率分别为(25.47±0.74)%,(28.08±1.41)%和(35.49±0.66)%,与对照组(21.26±0.70)%比较,差异有统计学意义(P<0.01);随药物浓度增加,核固缩浓染的凋亡细胞明显增多,COX-2和stathmin蛋白表达下调。结论:五倍子提取物鞣花酸有抗鼻咽癌细胞的作用,其机制可能与COX-2和stathmin下调相关。
Objective： To determine the biological activity of ellagic acid extracted from gallnut against nasopharyngeal carcinoma and its molecular mechanism. Methods： Nasopharyngeal carcinoma 5-8F cells were treated with 2, 4, 6 μg/mL ellagic acid for 48 h in vitro. The cell proliferation and cell apoptosis were analyzed by Mq-r and Hoechst33258 stain. The cell cycle and protein expression were measured by flow cytometry and Western blot. Results： Ellagic acid inhibited the proliferation of 5-8F cells. The inhibition rates were （29.35±4.95）%, （53.32 ±4.44）% and （ 61.75 ± 6.93）%, respectively, with significant difference from the control group （P〈0.01）. S phase cells in the experimental groups were （25.47±0.74）% （28.08±1.41）% and （35.49±0.66）%, respectively, with significant difference （P〈0.01） from the control group （21.26±0.70）%. Cells in the experimental groups showed nuclear pyknosis, karyorrhexis and poptotic cell morphology,The expression of COX-2 and stathmin in 5-8F cellswas down-regulated with increased drug concentration. Conclusion： Ellagic acid extracted from gallnut has activity against nasopharyngeal carcinoma cells, and its mechanism may be related to down-regulated expression of COX-2 and stathmin.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2012年第9期871-875,共5页
Journal of Central South University ：Medical Science
基金
广西中医药管理局项目(GZKZ09-72)
广西自然科学基金项目(2010GXNSFC013024)
广西教育厅项目(200911MS166)
教育部留学服务中心项目~~
