摘要
目的：研究沙门菌诱导小鼠产生肿瘤坏死因子。方法：将沙门菌SR-11分成两部分,一部分加热杀死,配成1×10^9cfu/鼠,称为死菌;另一部分活菌配成（5-6）×10^3cfu/鼠。结果：死菌注射后1小时,沙门菌抵抗鼠A/J在血清中产生大量的TFN-α;敏感鼠产生低剂量的TNF-α;腹水中出现低剂量的TNF-α。用活菌刺激小鼠仅在腹水中检出低剂量的TNF-α,血清中测不到。结论：用死菌刺激小鼠,会在血清中产生大量的TNF-α。
Objective：To study tumor necrosis factor （TNF） of mice induced by salmonellosis. Methods： Salmonellosis SR-11 were divided into two parts .The concentration of the dead bacteria is 109cfu/mice, the live bacteria 5-6 × 10^3cfu/mice. Results： One hour after injection salmonellosis-resist mice A/J could produce a great amount of TNF-α ; salmonellosis-sensitive mice could produce low level of TNF- α ; low level in peritoneal exudates. Only small amount of TNF- α in peritoneal exudates can be detected when the mice were stimulated by live bacteria. Conclusions： Great amount of TNF-α in serum can be detected after stimulated by dead bacteria..
出处
《中国伤残医学》
2012年第9期23-25,共3页
Chinese Journal of Trauma and Disability Medicine
关键词
TNF-Α
沙门菌
鼠血清
腹水
TNF- α
salmonellosis
Mice serum
Peritoneal exudates
