摘要
目的分析IL-12及SCF对CD34+细胞分裂增殖及IL-12Rβ的表达。方法分离正常足月产新生儿脐带血与健康成年人外周血中的单个核细胞,采用流式细胞术(FACS)比较2组中CD34+细胞的频率,分析IL-12及SCF对CD34+细胞的作用。结果健康成年人外周血中CD34+细胞平均频率为0.13%,显著低于新生儿脐带血中CD34+细胞的平均频率1.53%(P<0.001)。进一步研究表明,SCF可以促进CD34+细胞表达CD25;IL-12及SCF可以显著促进CD34+细胞的分裂增殖以及IL-12Rβ1的表达。结论新生儿脐带血中CD34+细胞的频率明显高于成年人外周血中CD34+细胞的频率。IL-12及SCF可以促进CD34+细胞的分裂增殖以及IL-12Rβ1的表达。
This paper aimed to analyze the roles of IL-12 and SCF on the cell divisions and IL-12Rβ expression of CD34＋ cells.The mononuclear cells were isolated from neonatal umbilical cord blood（CBMC） and adult peripheral blood（PBMC）.Then the frequency of CD34＋ cells from CBMCs and PBMCs were detected by FACS.Finally,we analyzed the effects of IL-12 and SCF on CD34＋ cells.About 0.13% of CD34＋ cells in PBMCs and 1.53% of CD34＋ cells in CBMCs were detected.The frequency of CD34＋ cells from CBMCs was higher than that from PBMCs.Furthermore,SCF could increase the expression of CD25 on CD34＋ cells.In conclusion,IL-12 and SCF could increase cell divisions and expression of IL-12Rβ1 on CD34＋ cells.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2012年第10期833-836,共4页
Immunological Journal
