摘要
目的研究活性牡蛎壳天然纳米体复合型骨材料作为骨缺损修复材料的可行性。方法 a)研制人工骨材料,并用扫描电镜观察其结构。b)把研制的牡蛎壳复合型骨材料、医用硫酸钙骨材料和自体骨植入人工形成的兔桡骨缺损区域内,进行体内生物相容性实验,通过对实验侧和对照侧进行大体标本X线片观察和组织学的检测,观察新骨形成、骨愈合、生物相容性等情况,评价该材料的骨缺损修复能力。结果天然牡蛎壳纳米体复合型骨材料多孔支架的孔径、孔隙率和抗压强度分别为200～400μm、40%～50%、45～49 M Pa。在2、4、8、12周进行组织学检查及X线检查,观察显示骨缺损修复程度,实验组优于医用硫酸钙组,生物力学测试显示术后12周实验组与自体骨比较抗弯曲强度无明显差别。结论天然牡蛎壳纳米体复合型骨材料相对医用硫酸钙有着更好的生物学特性、骨诱导性、成骨能力,并能更持久的提供支架作用修复骨缺损,在骨组织修复材料方面有应用前景。
Objective To explore the feasibility of active oyster shells natural nano body structured bone material as bone defect materials.Methods a）New artificial bone material was developed and the structure was studied by scanning electron microscopy.b）The materials was implanted in the rabbit radial defects for in vivo experiment.X-ray observation and histological study were performed.Results The aperture,porosity and compressive strength of porous scaffolds of natural oyster shells nano body structured bone material were 200～400 μm,40～50%,45 to 49 MPa.Histological examination and X-ray observations showed bone defect repair respectively was better than groups of medical calcium sulfate in 2,4,8,12 weeks..Biomechanical test showed no difference of compressive strength between the experimental group and autologous transplantation group after 12 weeks.Conclusion Natural oyster shell nano composite type bone material compared with medical calcium sulfate has better biological characteristics,osteoinductivity,osteogenesis effect and more durability to provide support in the repair of bone defect.
出处
《实用骨科杂志》
2012年第9期807-812,共6页
Journal of Practical Orthopaedics
基金
温州市科技计划项目(Y20090288)
关键词
牡蛎壳纳米体
人工骨
桡骨缺损
oyster shells natural nano body composited bone material； atificial bone； radius defect
