摘要
根结线虫卵的寄生真菌对根结线虫病害的生物防治具有很大的应用潜力。本研究采用卵寄生真菌刀孢蜡蚧菌Lecanicillium psalliotae CGMCC5329对南方根结线虫卵和雌虫的寄生性进行了测定,同时利用该菌的生物菌剂对烟草根结线虫病害进行了田间防效测定。结果表明,该菌能寄生根结线虫的卵和雌虫。其中,培养5 d对卵寄生率为83.29%;培养15 d对卵块相对孵化抑制率为91.41%。刀孢蜡蚧菌菌剂在田间施用15 kg/667m2时,相对防效达80%以上,刀孢蜡蚧菌CGMCC5329对烟草根结线虫病害具有较好的防治效果。
Egg-parasitic fungi have been investigated as a potentially useful biological control agent against root-knot nematodes. In these study, the parasitism of tobacco root-knot nematode eggs, female and the inhibition in hatch of egg masses were measured in vitro, and the biocontrol potential on the tobacco root-knot nematodes in the field were evaluated by Lecanicillium psalliotae CGMCC5329. The fungus colonized the root-knot nematodes eggs, females, and demonstrated the great suppression in hatch of egg masses. The egg parasiting rate by the fungus was 83.29% on the 5th day; the inhibition in hatch of egg masses was 91.41% on the 15th day. The relative effect control of tobacco root-knot nematodes was over 80% in the field with the fungal agent applying volume of 15 kg/667m2. This study suggests that L. psalliotae CGMCC5329 has great potential use for biological control against root knot nematodes.
出处
《中国烟草科学》
CSCD
2012年第4期65-69,共5页
Chinese Tobacco Science
基金
中国烟草总公司资助项目(110200902065-4
110200902067
110200801038
110200801007)
山东省烟草专卖局(公司)资助项目(201001)
关键词
刀孢蜡蚧菌
根结线虫
寄生性
烟草
田间防效
Lecanicillium psalliotae
rooto-knot nematode
parasitism
tobacco
field control
