摘要
目的:研究穿山龙总皂苷(TSRDN)对CIA大鼠滑膜血管新生的影响及其作用机制。方法:建立CIA大鼠模型,造模后给予TSRDN、雷公藤和薯蓣皂苷元灌胃,并采用免疫组化检测滑膜组织MVD、VEGF及Flk-1表达。结果:CIA模型组大鼠滑膜新生血管增多,MVD、VEGF及Flk-1表达升高(P<0.01),给药后大鼠滑膜新生血管数量减少,MVD、VEGF和Flk-1表达降低(P<0.01)。结论:穿山龙总皂苷可能通过抑制VEGF和Flk-1的表达来发挥抗血管新生作用。
Objective：To study effects of total saponin of Rhizoma Dioscreae Nipponicae（TSRDN） on angiogenesis in synovial tissue of CIA rats,and investigate the mechanism of TSRDN on antiangiogenesis in treating rheumatoid arthritis.Methed： After the CIA rat model was established,the rats in treatment group were lavaged with TSRDN,tripterygium,Diosgenin.Immunohistochemical staining was used to observe MVD,VEGF and Flk-1.Results： MDV,VEGF and Flk-1 expression of CIA model group were obviously higher than normal group（P0.01）.After treatment,MDV,VEGF and Flk-1 expression were obviously lower than CIA model group（P0.01）.Conclusions： TSRDN can inhibit angiogenesis in synovial tissue by down regulating VEGF and Flk-1 expression.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2012年第9期986-988,991,共4页
JOURNAL OF BASIC CHINESE MEDICINE
基金
国家自然科学基金资助项目(30873420)
