摘要
目的利用组织芯片技术结合免疫组化方法探讨RASSF1A和Elf-1基因在非小细胞肺癌(NSCLC)组织中的蛋白表达及与临床病理学特征的关系。方法构建含有72例非小细胞肺癌及10例正常肺组织标本的组织芯片,以免疫组织化学PowerVisionTM-900(PV-9000)法检测RASSF1A和Elf-1在NSCLC与正常肺组织中的蛋白表达。结果 RASSF1A和Elf-1在肺癌组织中均呈异质性表达。RASSF1A在NSCLC组织中的阳性率为47.22%(34/72),显著低于正常肺组织100%(10/10),两组间的蛋白表达差异具有统计学意义(P=0.002);RASSF1A在有淋巴结转移组的表达强度显著低于无淋巴结转移组(χ2=4.379,P=0.036);RASSF1A的表达在不同的临床分期间差异有统计学意义(χ2=17.979,P=0.000)。Elf-1在NSCLC及正常肺组织中的阳性表达率分别为73.61%(53/72)及20.00%(2/10),两组间的表达差异具有统计学意义(P=0.001),其表达强度与NSCLC的分化程度有关(χ2=7.116,P=0.028);有淋巴结转移组Elf-1的表达强度高于无淋巴结转移组(χ2=5.304,P=0.021);Elf-1的表达在不同的临床分期间亦有差异(χ2=6.791,P=0.034)。NSCLC组织中RASSF1A和Elf-1蛋白的表达强度呈负相关(r=-0.433,P=0.000)。结论在NSCLC组织中RASSF1A表达下调和Elf-1过度表达可能与NSCLC的发生、发展及预后不良有关,RASSF1A及Elf-1基因表达的检测有望作为NSCLC早期诊断、评估其侵袭性及转移性的重要参考指标。
Objective To explore the correlation between the expression of RASSF1A and Elf-1 proteins and clinicopathological features of non-small cell lung cancer（ NSCLC ）. Methods A tissue chip array containing 72 NSCLC tissue samples and 10 normal lung tissue samples was constructed,and immunohistochemistry （PV9000）was used to measure the expression of RASSF1A and Elf-I pro- teins. Results Both RASSF1A and Elf-1 were heterogeneously expressed in NSCLC tissue. RASSF1A protein was expressed in 34/72 （47.22%） of NSCLC tissues,a significantly lower proportion than the 10/10（100%） of normal lung tissues expressing RASSFIA.In addition, RASSF1A protein levels differed significantly between NSCLC tissue and normal lung tissue（P=0.002）.For example, RASSF1A protein levels were significantly higher in cases with lymph node metastasis than in cases without it（x2=4.379,P=0.036）. Signifieant differences were also observed between groups at different clinical stages（x2= 17.979,P=0.000）.Elf-1 protein was detected in 53/72（73.61% ） of NSCLC tissues but in only 2/10（20%） of normal lung tissues.Elf-1 protein levels differed significantly between the two types of tissue（P=0.001 ）.Expression of Elf-1 was related to tumor differentiation （X2=7.116,P=0.028）,lymph node metastasis （X2=5.304, P=0.021 ） and clinical staging（x2=6.791, P=0.034）.Levels of RASSF1A and Elf-1 proteins correlated negatively with each other in NSCLC tissue（r=-0.433,P=0.000）. Conelusions The down-regulation of RASSF1A and up regulation of Elf-1 may be asso- ciated with the occurrence,progression and prognosis of NSCLC.The relative expression of RASSF1A and Elf-1 may play an impor- tant role in early diagnosis of NSCLC,and serve as a molecular indieator for predicting invasion and metastasis.
出处
《中国癌症防治杂志》
CAS
2012年第3期246-251,共6页
CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT
基金
烟台市科学技术发展计划项目(2008162)
