摘要
目的:测定重症EV71脑炎患儿脑脊液和血清中神经元特异性烯醇化酶(NSE)、髓鞘碱性蛋白(MBP)和S-100β蛋白的含量,探讨其在EV71脑损伤中的评估价值。方法:采用ELISA法测定25例EV71脑炎患儿(脑炎组,重症16例,危重症9例)急性期脑脊液和血清中NSE、MBP、S-100β蛋白含量,并与10例健康儿童(对照组)进行比较。结果:(1)血清与脑脊液的NSE、MBP、S-100β含量经直线相关分析,r值分别为0.806,0.671,0.802(均P<0.01);(2)脑炎组血清NSE(7.46±2.74)ng/mL、S-100β(529.85±192.90)pg/mL,显著高于对照组血清NSE(4.13±0.68)ng/mL、S-100β(325.61±63.14)pg/mL(均P<0.01),脑炎组血清MBP(1.45±0.79)ng/mL与对照组血清MBP(1.22±0.56)ng/mL的含量差异无显著性(P=0.409);(3)与对照组相比,危重症组血清NSE、MBP和S-100β含量显著增高,NSE(10.34±1.50)ng/mL(P<0.01),MBP(1.95±0.48)ng/mL(P<0.05),S-100β(744.31±101.57)pg/mL(P<0.01),重症组血清NSE、S-100β含量增高,NSE(5.80±1.70)ng/mL(P<0.05)、S-100β(409.22±104.50)pg/mL(P<0.05)。(4)与重症患儿相比,危重症患儿血清NSE、MBP、S-100β含量明显增高(均P<0.01)。结论:NSE、MBP、S-100β水平与病情严重程度有关,检测其血清含量有助于EV71感染后脑损伤的病情评估。
Objective To measure the levels of neuron specific enolase （NSE）, myelin basic protein （MBP）, and S-100βprotein in cerebrospinal fluid （CSF） and serum in children with EV71 encephalitis, and to study their value in the evaluation of EV71-related brain injury. Methods The levels of NSE, MBP and S-100βprotein in CSD and sera in 25 patients with severe EV71 encephalitis （16 were severe cases, 9 were critically ill cases） and 10 normal heahhy controls. Results The levels of NSE, MBP and S-100β protein in serum were positively correlated with those in CSF （r = 0.806, 0.671, 0.802, respectively, all P 〈 0.01）. As compared to those in control group, the levels of NSE and S-100β protein in the encephalitis group were significantly increased [ （7.46 ± 2.74） ng/mL vs. （4.13 ± 0.68） ng/mL; （529.85 ± 192.90） pg/mL vs. （325.61 ± 63.14） pg/mL, P 〈 0.01]. There was no significant difference in the level of MBP in serum between the two groups [ （ 1.45 ± 0.79） pg/mL vs. （1.22 ± 0.56） pg/mL, P = 0.409]. Significant increases of the levels of NSE [（10.34 ± 1.50） ng/mL], MBP [（1.95 ±0.48） pg/mL] and S-100β[（744.31 ± 101.57） pg/mL] in srum were observed in the critically ill group （P 〈 0.01, P 〈 0.05 ,P 〈 0.01, respectively）, while those of NSE [ （5.80 ± 1.70） ng/mL] and S-100β [ （409.22 ± 104.50） ng/mL] in serum in severe group were significantly increased （both P 〈 0.05 ）. As compared to those in the severe group, the levels of NSE, MBP and S-100β in serum in critically ill group were higher （all P 〈 0.01 ）. Conclusions Levels of NSE, MBP and S-100β were correlated well with the severity of EV71 encephalitis, and their serum levels may help to evaluate the condition of brain injure after EV71 infection.
出处
《实用医学杂志》
CAS
北大核心
2012年第19期3192-3195,共4页
The Journal of Practical Medicine
基金
广东省卫生厅指令性课题(编号:C2010020)
