摘要
目的:探讨信号转导及转录激活因子1(signal transducer and activator of transcription 1,STAT1)和细胞间黏附分子1(intercellular adhesion molecule-1,ICAM-1)在非小细胞肺癌(non-small-cell lung cancer,NSCLC)中的表达及其临床意义。方法:采用real-time PCR和Western blotting方法,检测STAT1和ICAM-1在非小细胞肺癌组织中的表达,并比较二者与临床多种因素(包括病理类型、分化程度、TNM及淋巴结转移)的关系,以40例肺部非恶性组织标本(癌旁正常肺组织)为对照。结果:肺癌组织中STAT1及ICAM-1的表达明显高于其在癌旁肺组织中的表达(P<0.05)。ICAM-1在腺癌中的表达高于鳞癌(P<0.05),在有淋巴结转移时比无转移时增强(P<0.05)。STAT1的表达在肺癌早期组高于肺癌晚期组(P<0.05)。ICAM-1的表达在肺癌晚期组高于肺癌早期组(P<0.05)。STAT1在低分化肺癌组织中的表达比在高-中分化组织中增强(P<0.05)。在不同年龄、性别的肺癌组织中STAT1和ICAM-1的表达没有显著差异。结论:STAT1及ICAM-1在非小细胞肺癌中高表达,且其表达与肺癌的侵袭转移、不同分期和病理类型密切相关。
To investigate the clinical significance of signal transducer and activator of transcription 1 （ STAT1 ） and intercellular adhesion molecule - 1 （ ICAM - 1 ） in non - small - cell lung cancer （NSCLC）. METHODS ： The expression of STAT1 and ICAM - 1 at mRNA and protein levels was detected by real - time PCR and Western blotting in the fresh samples from 40 cases of NSCLC to analyze the correlation between STAT1, ICAM - 1 and clinical characteris- tics （ sex, age, histological type, cell differentiation, TNM stage and distant metastasis）. Forty cases of non - malignant tissues from lung lesion （adjacent normal lung tissues） were used for control. RESULTS： STAT1 and ICAM - 1 were evi- dently and more strongly expressed in lung cancer than non - malignant tissues （ P 〈 0. 05 ）. In lung cancer, the expression of ICAM - 1 in adenocarcinoma was stronger than that in squamous cell carcinoma. ICAM - 1 expression in lung cancer with lymph node metastasis was stronger than that in lung cancer without lymph node metastasis （ P 〈 0. 05）. STAT1 ex- pression in lung cancer in the early stage was stronger than that in the late stage. ICAM - 1 expression in lung cancer in the late stage was stronger than that in early stage. STAT1 expression in poorly differentiated cancer was also stronger than that in well differentiated one （P 〈 0. 05）. The expression of STAT1 and ICAM - 1 was not correlated with age and sex （ P 〉 0.05 ）. CONCLUSION ： The expression levels of STAT1 and ICAM - 1 are closely related to the invasion, metastasis and different stages of lung cancer.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2012年第8期1378-1382,共5页
Chinese Journal of Pathophysiology
基金
温州市科技计划项目(No.H20080058)
关键词
肺肿瘤
信号转导及转录激活因子1
细胞间黏附分子1
Lung neoplasms
Signal transducer and activator of transcription 1
Intercellular adhesion mole-cule - 1
