摘要
目的:用HPLC法建立钩藤药材中钩藤碱(Rhynchophylline)、异钩藤碱(Isorhynchophylline)的含量测定方法,用于钩藤药材的质量控制。方法:色谱柱为Diamonsil C18柱(4.6 mm×250 mm,5μm),以甲醇:水=65:35(V/V,含0.01mmol/L三乙胺,冰醋酸调PH7.0)为流动相,流速为1.0 ml.min-1,在245nm检测波长下,测定钩藤药材中钩藤碱、异钩藤碱含量。结果:钩藤碱及异钩藤碱的回归方程分别为Y=1.0E+6x-21142和Y=1.1E+6x-13566,线性范围分别为0.254～1.524μg和0.216～1.296μg,相关系数分别0.9999和0.9999。结论:本法正确、简便、快速,对于控制和评价钩藤药材的品质有重要意义。
Objective ： To determine the contents of Rhynchophylline and Isorhynchophylline in Uncaria rhynchophylla （ Miq. ） Jacks. by the method of HPLC . Methods ： Contents of the isoflavones （ Rhynchophylline and Isorhynchophylline） in Uncaria rhyn- chophylla （ Miq. ） Jacks. was measured by HPLC [ a column of Diamonsil C18 （ 4.6 mm x 250 mm, 5 um ）, methanol： water = 65 ： 35 （ V ! V, with 0. 01 mmol ! L triethylamine, glacial acetic acid adjusted PH7.0） as the mobile phase, flow rate 1.0 mL ~ min - 1, detective wave 245 nm] to determine the contents of Rhynchophylline and Isorhynchophylline. Results ： The two regression e- quations are Y = 1.0E ＋ 6x - 21142 and Y = 1.1E ＋ 6x - 13566, and have good linearity in the range of 0. 254 - 1. 524 ug and 0. 216 - 1. 296 ug , r =0. 9999 and 0. 9999 respectively. Conclusion： The method is correct , simple, rapid, it is vital for control- ling and evaluating the quality of Chinese medicine Uncaria rhynchophylla （ Miq. ） Jacks.
出处
《中国民族民间医药》
2012年第18期56-57,共2页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
国家"重大新药创制"科技重大专项-钩藤降压缓释制剂的研制
关键词
钩藤
高效液相色谱法
钩藤碱
异钩藤碱
Uncaria rhynchophylla （Miq.） Jacks.
HPLC
Rhynchophylline
Isorhynchophylline
