摘要
为了对水貂阿留申病毒的诊断防治提供依据,验证水貂阿留申病毒在我国的遗传演化情况,研究了阿留申病毒的诊断抗原。将疑似阿留申病毒感染致死的送检水貂进行PCR初步诊断,并应用猫肾传代细胞系(CRFK)进行了分离培养。提取细胞培养物的DNA,经PCR检测呈阳性后,对分离前后的阿留申病毒VP2基因全序列进行克隆测序,其结果与GenBank上传的其它ADV亚型VP2序列进行了比较后证明:获得了一株可以在CRFK细胞中稳定生长的水貂阿留申病毒毒株(命名为ADV-ZYL1)。氨基酸分析发现,ADV-ZYL1毒株与美国强毒力毒株ADV-Utah-1的氨基酸同源性最高,为96.1%,与ADV-Far East的氨基酸同源性为92.7%,表明ADV-ZYL1的VP2蛋白氨基酸存在很高的突变率。
An experiment was conducted to study the genetic evolution types of Aleutian mink disease parvovirus in order to pro- vide more conveniences for the future researches of ADV antigen in China. The samples collected from minks, which were suspected of being infected with ADV, were amplified using PCR method for a primary diagnosis, and the viruses were extracted and serially passaged in Crandell Feline Kidney cells （CRFK）. If a PCR amplification indicated a positive result, the VP2 gene sequences before and after separation would be sequenced. The results were compared with other ADV VP2 gene sequences obtained from the GenBank. A strain of ADV, which could grow normally in CRFK cells （ named ADV- ZYL1 ）, was obtained. Amino acid sequence analysis indicates that the VP2 protein sequence of the ADV-ZYL1 strain has a highest homology of 96.1% with ADV-Utah-1, which is a highly pathogenic strain in USA; while it has only a homology of 92.7% compared with ADV-Far East, indicating that the VP2 protein of ADV-ZYL1 presents a high mutation rate.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2012年第9期97-100,共4页
Journal of Northeast Forestry University
基金
国家林业局野生动植物保护与自然保护区管理司项目资助
关键词
水貂阿留申病毒
VP2
分离
鉴定
Aleutian mink disease parvovirus
VP2 genes
Isolation
Identification
