摘要
目的:建立用高效液相色谱法测定丹参口服液中丹参素、原儿茶醛含量的测定方法。方法:采用HYPERSIL ODS2(4.6mm×200.0 mm,5μm)色谱柱,流动相为甲醇-1%醋酸(1:7),检测波长:280 nm。结果:丹参素的线性范围为:0.952 6～2.222 6μg,原儿茶醛的线性范围为:0.078 7～0.183 7μg;加样回收率:丹参素为99.84%,RSD为0.95%(n=5),原儿茶醛为99.91%,RSD为2.17%(n=5)。结论:高效液相色谱法操作简便、准确、重现性好,便于控制产品质量。
Objective：To establish assay method for the determination of propanoid acid and protocatechualdehyde in Salvia miltiorrhiza oral liquid by HPLC. Methods：Use HYPERSIL ODS2 （4.6 mm×200.0 mm,5μm） column as chromatographic column and a mixture of methanol and 1% acetic acid（ 1 ： 7 ） as mobile phase. As detector a spectrophotometer set at 280 nm. Results：Linear range of pro- panoid acid ：0.952 6 - 2. 222 6μg. Linear range of protocatechualdehyde ：0.078 7 - 0. 183 7μg. Spotting coefficient of recovery of pro- panoid acid is 99.84% and RSD is 0.95% （n = 5 ）, protocatechualdehyde is 99.91% and 2.17% （n = 5 ） separately. Conclusion： HPLC method is simple, accurate,reproducible and easy to control the quality of products.
出处
《中医学报》
CAS
2012年第9期1155-1156,共2页
Acta Chinese Medicine
基金
国家自然科学基金项目(编号:50827530)
关键词
丹参口服液
高效液相色谱法
丹参素
原儿茶醛
产品质量
salvia mihiorrhiza oral liquid
high performance liquid chromatography
propanoid acid
protocatechualdehyde
product qual-ity
