摘要
目的建立同时测定氨咖黄敏胶囊中三组分(对乙酰氨基酚、咖啡因、马来酸氯苯那敏)的高效液相色谱法。方法色谱柱为Agilent TC-C18(4.6 mm×150 mm,5μm);流动相为磷酸盐缓冲液(取磷酸二氢铵11.5 g,加水适量使溶解,加磷酸1 mL,用水稀释至1 000 mL)-乙腈(85∶15);流速为1.0 mL·min-1;检测波长为225 nm;柱温为30℃。结果对乙酰氨基酚、咖啡因和马来酸氯苯那敏的进样量分别在2.523～7.569,0.149 6～0.448 8和0.010 08～0.03024μg范围内与峰面积呈良好线性关系,r值分别为0.999 8,0.999 9,0.999 9,平均回收率分别为99.6%,99.5%,99.8%,RSD分别为0.56%,0.60%,0.65%(n=9)。结论本方法简便、快速、准确,可用于氨咖黄敏胶囊的质量控制。
Objective To establish an HPLC method for the determination of three components （ paracetamol, caffeine and chlorphenamine maleate） in Ankahuangmin Capsules. Methods The analytical column was Agilent TC - C18 （4.6 mm× 150 mm,5 μm）. The mobile phase was phosphate buffer （ 1.5 g ammonium phosphate monobasic was dissolved and 1.0 mL phos- phoric acid was added in water diluted to 1 000 mL） -acetonitrile（85： 15） and the flow rate was 1.0 mL · min-1. The detection wavelength was 225 nm and the column temperature was 30 ℃. Results The linear ranges of paracetamol, caffeine and chlor- phenamine maleate were 2.523 - 7. 569,0. 149 6 - 0.448 8 and 0.010 08 - 0. 030 24 μg with relative coefficient of r = 0. 999 8, r=0. 999 9,r =0.999 9 and relative average recoveries of 99.6% （RSD =0. 56% ,n =9） ,99.5% （RSD =0. 60% ,n =9） and 99.8% （RSD = 0. 65 % ,n = 9） ,respectively. Conclusion The method was simple, rapid and accurate,and it can be used for the quality control of Ankahuangmin Capsules.
出处
《齐鲁药事》
2012年第8期462-463,465,共3页
qilu pharmaceutical affairs
