摘要
采用简单的"胶原酶直接消化法"分离鹅胚胎肝细胞,得到的肝细胞纯度高,活性好;HE染色显示大量双核或多核细胞,细胞具有旺盛增殖分裂能力;肝脏特异性基因白蛋白基因检测显示所分离细胞为成熟肝脏细胞。该法操作简单、高效,是获得鹅原代肝细胞的一种较好的方法,有利于以鹅原代肝细胞为模型的体外研究工作的开展。
Using the simple method of collagenase digestion directly＇to isolate goose embryo hepatocytes and can acquire a large high viability and high purity of hepatocytes. HE dyed showed that mostly hepatocytes have two or more nucleus which demonstrate cells have strong proliferation split ability . The specific gene albumin gene test showed that isolated cells are mature hepatocytes. It is a easily and effective method which can get primary goose hepatocyte.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2012年第9期1378-1380,1385,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31072021)
江苏省教育厅重大基础研究资助项目(10KJA430054)
江苏省高校自然科学基金资助项目(11KJB230005)
关键词
鹅胚胎
鹅原代肝细胞
白蛋白
goose embryo
primary goose hepatocyte
albumin
