摘要
目的探讨凋亡抑制蛋白Bcl-2、凋亡促进蛋白Hrk及细胞周期素E(Cyclin E)在胃癌中的表达及其意义。方法采用免疫组化(S-P)法检测58例胃癌患者的癌组织蜡块标本及正常癌旁组织中Bcl-2蛋白、Hrk蛋白及Cyclin E蛋白的表达情况。结果 Bcl-2蛋白在胃癌组织中阳性表达38例,阳性率为65.5%,癌旁组织中阳性表达14例,阳性率为24.1%,两种组织中Bcl-2蛋白阳性率比较,差异有统计学意义(χ2=20.077,P<0.05)。不同肿瘤分化程度、临床分期及有无淋巴转移胃癌患者中Bcl-2蛋白阳性率比较,差异均有统计学意义(P<0.05);不同年龄、肿瘤直径、Borrmann分型、浸润深度胃癌患者中Bcl-2蛋白阳性率比较,差异均无统计学意义(P>0.05)。Hrk蛋白在胃癌组织中阳性表达12例,阳性率为20.7%,癌旁组织中阳性表达36例,阳性率为62.1%,两种组织中Hrk蛋白阳性率比较,差异有统计学意义(χ2=20.471,P<0.05)。Cyclin E蛋白在胃癌组织中阳性表达34例,阳性率为58.6%,癌旁组织中阳性表达11例,阳性率为18.9%,两种组织中Cyclin E蛋白阳性率比较,差异有统计学意义(χ2=19.206,P<0.05)。Spearman相关分析表明,Bcl-2蛋白与Hrk蛋白呈负相关(r=-0.286,P<0.05),与Cy-clin E蛋白呈正相关(r=0.749,P<0.05)。结论胃癌的形成与多种蛋白的相互作用及基因水平上正负信号转导、调控密切相关,凋亡抑制蛋白Bcl-2、凋亡促进蛋白Hrk及Cyclin E共同参与胃癌的发生发展。
Objective To study the expression and significance of inhibitor of apoptosis protein Bcl -2, apoptosis promote protein Hrk and cell cycle element Cyclin E in gastric cancer. Methods The expressions of Bcl -2, Hrk and Cyclin E in the samples of gastric cancer and adjacent normal tissues of 58 patients with gastric cancer were detected by S - P meth- od. Results Bcl -2 protein had positive expression in 38 cases of gastric cancer tissues with a positive rate of 65.5%, and had positive expression in 14 cases of adjacent normal tissues with a positive rate of 24. 1%. The difference of positive rate between the two tissues showed statistically significant difference （ X2 = 20. 077, P 〈 0. 05 ） . Patients with different cancer differentiation lev- els, clinicM stages and whether having lymph node metastases or not showed statistically significant difference in the positive rate of Bcl - 2 protein （P 〈 0. 05 ） . Patients with different age, tumor diameter, Borrmann type and infiltration depth showed no sta- tistically significant difference in the positive rate of Bcl -2 protein （P 〉 0. 05 ） . Hrk protein had positive expression in 12 cases of gastric cancer tissues with a positive rate of 20. 7%, and had positive expression in 36 cases of adjacent normal tissues with a positive rate of 62. 1%. The difference of positive rate between the two tissues showed statistically significant difference （ x2 = 20. 471, P 〈0. 05） . Cyclin E protein had positive expression in 34 cases of gastric cancer tissues with a positive rate of 58.6%, and had positive expression in 11 cases of adjacent normal tissues with a positive rate of 18.9%. The difference of positive rate be- tween the two tissues showed statistically significant difference （ X2 = 19. 206, P 〈0. 05） . Spearman correlation analysis showed that Bcl - 2 protein was negatively correlated with Hrk protein （ r = - 0. 286, P 〈 0. 05 ）, and was positively correlated with Cy- clin E protein （ r = 0. 749, P 〈 O. 05 ） . Conclusion The path
出处
《中国全科医学》
CAS
CSCD
北大核心
2012年第24期2790-2793,共4页
Chinese General Practice
基金
新疆生产建设兵团卫生科技计划基金项目(2011年第16号)
