摘要
目的探讨血管内皮生长因子(VEGF)基因多态性与支气管肺发育不良(BPD)相关性。方法选自2009-07-01—2012-02-01华中科技大学同济医学院附属同济医院收治的符合BPD诊断标准的患儿64例(BPD组)和同期住院的无肺部疾病患儿106例(对照组)。利用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法分析两组VEGF+405、-2578基因频率分布,酶联免疫吸附试验(ELISA)分析BPD组血浆VEGF浓度。结果 (1)VEGF+405G>C:BPD组中G的基因型频率和等位基因频率均高于对照组,差异有统计学意义(P<0.05);VEGF-2578C>A:BPD组中A的基因型频率和等位基因频率均高于对照组,差异有统计学意义(P<0.05)。(2)BPD组VEGF+405GG/GC血浆表达低于VEGF+405CC(P<0.05),VEGF-2578CC/CA血浆表达与VEGF-2578AA差异无统计学意义(P>0.05)。结论 VEGF+405、-2578基因多态性和BPD相关,推测其和BPD遗传易感性相关。VEGF+405多态性与VEGF血浆表达相关,血浆中VEGF减低可能是BPD发病机因素之一。
Objective To investigate the association between single nueleotide polymorphism of vascular endothelial growth faetor（VEGF）and bronchopulmonary dysplasia（BPD）. Methods A total of 64 BPD patients in BPD group and 106 patients without lung disease in control group from July 1,2009 to February 1,2012 were included in the study.Gen- otype of VEGF polymorphisms were performed by polymerase chain reaction and restriction fragment length po]ymorphism analysis （PCR-RFLP）. Enzyme-linked immunosorbent assay （ELISA）was used to detect the expression level of VEGF in plasma in BPD group. Results （ 1 ） VEGF ＋405G 〉 C： compared with control group, the genotype and allele frequencies of G was higher in BPD group with statistical difference （P 〈 0.05）. VEGF-2578C〉A： compared with con- trol group, the genotype and allele frequencies of A was higher in BPD group with statistical difference （P 〈 0.05 ）. （2） In BPD group the level of VEGF＋405 was lower in the patients with genotype GG（P 〈 0.05）compared with CC while there was no difference for VEGF-2578 with genotype CC and GG （P 〉 0.05）.Conclusion Single nucleotide polymorphisms of VEGF＋405 and VEGF-2578 could be associated with BPD, and VEGF＋405 influences its protein expression, and the lower VEGF level in plasma could he one of the factors for BPD pathogenesis.
出处
《中国实用儿科杂志》
CSCD
北大核心
2012年第9期682-685,共4页
Chinese Journal of Practical Pediatrics
基金
国家自然科学基金资助项目(No.30872795
No.81170001)
湖北省自然科学基金资助项目(No.2008CDB139)
新教师基金资助项目(高等学校博士点专项科研基金
No.200804871058)
关键词
血管内皮生长因子
单核苷酸多态性
支气管肺发育不良
遗传易感性
vascular endothelial growth factor
single nucleotide polymorphism
bronchopulmonary dysplasia
genetic predisposition
