摘要
用氯仿提取大鼠尿、粪标本中炎琥宁,并在硅胶F_(254)薄层板上点样。经氯仿—石油—乙醇—冰醋酸(6:3:0.5:0.5)展开。置薄层板于岛津双波长色谱扫描仪(CS-930型)内,以测定波长λ_=290nm,参比波长λ_R=360nm用反射吸收法进行锯齿描,测定大鼠尿、粪中炎琥宁含量。方法灵敏、准确、专属性好。
Yanhuning is a semisynthetic preparation of andrographolide. The chemical name of yanhuning is monopotassium anhydroandro-grapholide disuccinate (DASK). The urine and feces samples containing DASK were extracted with chloroform. The chloroform solutions were spotted on silica gel plates (F254) and developed with chloro form-petroleum ether-ethanol-glacial acetic acid (6:3:0.5:0.5). After development the plate was placed on a Shimadzu dualwavelength TLC scanner CS-930. A zigzag scanning was then carried out for each spot developed at s=290nm andR=360nm under ultraviolet reflection. The DASK in each spot was tdus quantitated. The re suits obtained in present paper indicate that the method is sensitive, accurate and specific and it is applicable for quuntitative analysis of DASK in biological samples
出处
《重庆医科大学学报》
CAS
CSCD
1990年第1期74-76,共3页
Journal of Chongqing Medical University
