摘要
背景：目前常用的Polycut硬组织切片机难以制作皮质骨或含坚硬植入物组织的薄层切片,近年来这些切片多用EXAKT硬组织切磨系统进行制作。目的：实验采用EXAKT切磨系统制作含金属内植物的软、硬组织薄层切片。方法：取大鼠股骨远端、小鼠股骨干骨折部、带有钛合金种植体的犬下颌骨和带有钴铬支架的猪冠状动脉标本,经多聚甲醛固定和丙酮脱水后用有机玻璃（甲基丙烯酸甲酯）浸透包埋。组织包埋块用EXAKT系统作厚度约300μm的切片,然后磨片至厚5~10μm的切片。不同切片用苏木精-伊红和甲苯胺蓝染色后在光学显微镜下观察形态。结果与结论：甲苯胺蓝染色显示,大鼠股骨远端骨组织切片中干骺端矿化骨小梁呈浅蓝色、皮质骨结构完整,无碎裂。含带有钛合金种植体的犬下颌骨切片浅蓝色宿主骨与黑色金属种植体密切结合,形成牢固的界面。四环素标记的小鼠股骨干骨折部骨痂切片,在荧光显微镜（紫外光）下显示明显的双层荧光带。苏木精-伊红染色的包裹钴铬合金支架材料的猪冠状动脉组织切片显示,支架材料维持在原位,与血管壁无脱离或游移现象,组织无皱缩或折叠。结果提示,采用EXAKT切磨系统技术能制作出高质量的软、硬组织薄层切片,可清晰显示各种组织结构和人工植入物。
BACKGROUND: It is difficult to cut thin sections of cortical bone and tissues installed with hard implant using Polycut microtome. Recently, such sections are commonly fabricated with EXAKT system. OBJECTIVE: To prepare thin sections of soft and hard tissues that contained metal implants by using EXAKT system. METHODS: The samples were obtained from the distal femur of rat, the fracture callus in mouse femur, the canine mandible with titanium alloy implant and the porcine coronary artery containing Co-Cr stent. The samples were fixed with polyformaldehyde and dehydrated with acetone, and then embedded with methylmethacrylate. The embedded samples were cut into 300 μm thick sections and then ground down to 5-10 μm. The thin sections were stained with hematoxylin-eosin or toluidine blue when necessary and observed under light microscope. RESULTS AND CONCLUSION: Toluidine blue staining showed that metaphyseal mineralized trabecular bone in the tissue sections of rat distal femur was light blue, and cortical bone was integrated with no fragmentation. The light blue host bone of the canine mandible with titanium alloy implant sections was closely integrated with the black metal implant and formed a solid interface. The callus sections of the tetracycline-labeled mouse femoral shaft fracture showed significant double fluorescence band under fluorescence microscope (ultraviolet light). Hematoxylin-eosin staining of the porcine coronary artery sections containing Co-Cr stent showed that the scaffold material remained in place without detachment or wavering phenomenon from the vessel wall, and the tissue was contained without shrinkage or collapse. The results suggest that proper use of EXAKT system is able to produce high-quality sections of soft and hard tissues, which can show the tissue structures and installed implant clearly.
出处
《中国组织工程研究》
CAS
CSCD
2012年第35期6555-6559,共5页
Chinese Journal of Tissue Engineering Research
基金
上海交通大学医学院基金(11XJ21035)
课题名称:骨质疏松性骨组织的形态学计量
无机物物质组分检测与 DEXA 测量间的相关性分析
上海市科委非政府间合作项目(10410702000)
课题名称:针对骨修复过程的关键环节构建多级联性促进骨修复的生物材料及其机理研究~~
