摘要
目的观察硫化氢（H2S）对兔心肺复苏后早期NSE、SIOOB以及海马神经元凋亡的影响。方法25只日本大耳白兔随机（随机数字法）分为3组：假手术组（X组）、心搏骤停组（CA组）和H2S处理组（H：S组）。兔吸入5％氟烷麻醉后，气管切开，右股静脉置管用于给药，右颈动脉穿刺置管用于血压监测和采血。CA组和H：S组夹闭家兔气管导管8min制备心搏骤停模型，并分别在恢复自主循环后吸入30％O：或体积分数为80×10^-6H2S。于夹闭气管导管前（基础值）及恢复自主循环后30min、60min采动脉血检测血浆中神经元特异性烯醇化酶（neuron．specificenolase，NSE）和SIOOB的质量浓度。恢复自主循环后60min处死家兔，取脑后分离海马固定于4％多聚甲醛中，待做组织病理学检查。计量资料以均数±标准差（x±s）表示，采用单因素方差分析，组间比较采用SNK—q检验，以P〈0．05为差异有统计学意义。结果与S组同时点比较，CA组和H2S组NSE和SIOOB的质量浓度均明显增高（P〈0．05），海马CA1区存活神经元减少，活化型caspase-3阳性神经元增多（P〈0．05）；与CA组比较，H：s组恢复自主循环后60min时S100B质量浓度显著降低（P〈0．05），海马CA1区存活神经元增多，活化型caspase-3阳性神经元显著减少（P〈0．05）。结论H2S抑制海马CA1区神经元凋亡，增加存活神经元数目，降低血浆中S100B的水平，进而减轻心搏骤停复苏后神经功能的损伤。
Objective To explore the effects of hydrogen sulfide （H2S） on neuron specific-enolase （NSE） , neurotrophic protein SI00B and neurons apoptosis in hippocampus CA1 region in the early stage of cardiopulmonary resuscitation （CPR） in rabbits. Methods Twenty-five Japanese white rabbits were randomly divided into three groups ： sham group （ S group）, cardiac arrest group （ CA group ） and H2 S treatment group （HES group）. Rabbits were anaesthetized with 5% halothane, trachea was exposed and intubated, right femoral vein was cannulated for medical agent administration, and right carotid artery was cannulated for monitoring of blood pressure and blood samples taken. Cardiac arrest was produced by suffocation with clamping the endotracheal tube and turning off mechanical ventilation. After 8 min of the endotracheal tube clamping, rabbits received CPR. After the restoration of spontaneous circulation （ROSC）, rabbits in groups CA and H2S inhaled 30% 02 or 30% O2 containing 80 × 10^-6 H2S,respectively. Blood samples were taken before, and 30 min and 60 rain after ROSC for detection of the concentrations of NSE and S100B in the plasma. As 60 min after ROSC, rabbits were decapitated after perfusion with 500 ml phosphate-buffered saline and followed by 4% paraformaldehyde 500 ml through aortic artery, and then the hippocampus was removed rapidly and fixed in 4% paraformaldehyde for the histological examination. All values were expressed in mean _＋ standard deviation （x± s）. Comparisons were carried out among different groups with SNK-q test of one-way analysis of variance （ One-Way ANOVA plus SNK）. Results In comparison with group S, the concentrations of NSE and S100B were significantly increased 30 rain and 60 min after ROSC （P 〈 0. 05）, the viable neurons were decreased and cleaved caspase-3 positive neurons in hippocampus CA1 region increased 60 min after ROSC in groups CA and H2S （P 〈 0. 05 ）. In comparison with group CA, the concentration of S100B decreased 60 rain afte
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2012年第9期987-991,共5页
Chinese Journal of Emergency Medicine
基金
基金项目：国家自然科学基金（81000822）
黑龙江省青年科学基金（QC2010056）
