摘要
目的探讨秋水仙素对在嗅感觉神经元分化增生过程中β-Ⅲtubulin的作用。方法孕鼠30只,随机分为秋水仙素组和正常组各15只。秋水仙素组E0.5d起每日尾静脉注射秋水仙素(0.1mg/kg),对照组每日予等量生理盐水尾静脉注射。通过双重免疫荧光检测,分别观察E9.5d、E11.5d、E14.5d、E17.5d胎鼠嗅上皮在分化过程中的形态变化,以及实时定量PCR方法检测嗅标志蛋白含量改变。结果秋水仙素处理后,不同发育时期胎鼠β-Ⅲtubulin及嗅标志蛋白表达均较同时期对照组明显减弱,且实时定量PCR检测嗅标志蛋白含量减低。结论秋水仙素作用于β-Ⅲtubulin蛋白,阻碍了嗅感觉神经元的发育与成熟。
Objective To investigate the role of colchicine on β-Ⅲ tubulin of olfactory sensory neurons differentiation and proliferation. Methods 30 pregnant mice were randomly divided into colchicine and control groups, and 15 mice in each group. Colchicine group was intravenously injected with E0.5 day of colchicine （0.1mg/kg）, and the control group was daily injected with saline. Olfactory epithelium hyperplasia in the process of differentiation was observed at E9.5, Ell.5, E14.5, E17.5 days using double immunofluorescence, and olfactory was detected using real-time quantitative PCR. Results after treatment with colchicines, β-Ⅲ tubulin and olfactory were decreased at the different stages of fetal development compared with the control group, and the real-time quantitative PCR data showed that olfactory also decreased. Conclusion Colchicine effect on β-mtubulin protein inhibits olfactory receptor neurons development and mature.
出处
《中国中西医结合耳鼻咽喉科杂志》
2012年第4期241-245,共5页
Chinese Journal of Otorhinolaryngology in Integrative Medicine
