摘要
目的探讨槲皮素及其糖苷衍生物在人小肠吸收模型Caco-2细胞单层上的吸收特征。方法采用Caco-2单层细胞模型,研究槲皮素、槲皮苷、异槲皮苷的肠道吸收特征,以LC-MS法测定槲皮素、槲皮苷、异槲皮苷、异鼠李亭。结果槲皮素及其糖苷衍生物孵育30～150min后,均能在透过侧检测到槲皮素、槲皮苷和异槲皮苷以及槲皮素的甲基化代谢产物之一异鼠李亭;透过的槲皮素及其代谢产物异鼠李亭含量在150min孵育时间内呈先升后降的趋势特征;而槲皮苷和异槲皮苷的透过量则随孵育时间呈持续升高的趋势,在孵育后期可检测到微量异鼠李亭。结论槲皮素、槲皮苷和异槲皮苷可以完整的分子形式被Caco-2细胞单层吸收,其吸收特征有显著差异,并在吸收过程中伴有广泛的代谢转化。[营养学报,2012,34(4):358-361,367]
Objective To investigate the intestinal absorption of quercetin and its glycosides.Method The absorption of quercetin,quercitrin,isoquercitrin and isorhamnetin were studied using Caco-2 cell monolayer by liquid chromatography-mass spectrometry（HPLC-MS） method.Results Quercetin,quercitrin,isoquercitrin and isorhamnetin were found in the receiver sides throughout 150 min incubation period.The contents of quercetin and isorhamnetin in the receiver sides reached to the peak volume at 120 min,and then decreased gradually.Meanwhile,the contents of quercitrin and isoquercitrin rose up in a linear manner during incubation.The isorhamnetin could also be detected.Conclusion Quercetin and its glycosides can transport wholly across the Caco-2 cell monolayer and subject to an extensive metabolism,but there is remarkable difference between them.
出处
《营养学报》
CAS
CSCD
北大核心
2012年第4期358-361,367,共5页
Acta Nutrimenta Sinica
基金
国家科技支撑项目(No.2008BAI58B06)
