摘要
【目的】研究耐旱性高的红麻品种在干旱胁迫条件下的蛋白质表达,揭示红麻耐旱性的生理机制。【方法】以鉴定出的耐旱性红麻品种GA42为材料,在苗期(五叶期)设置正常供水与控水比较试验,运用双向电泳分析红麻在干旱胁迫和正常供水条件下叶片蛋白质组的动态变化。【结果】对2-DE图谱分析后发现,在干旱胁迫下出现65个差异表达蛋白质点,选用表达量明显上调的9个蛋白质点,通过MALDI-TOF-TOF MS分析和数据库检索,鉴定出6个差异表达蛋白的功能,分别是2个核酮糖-1,5-二磷酸羧化酶(Rubisco)或其大亚基(所有植物进行光合碳同化的关键酶)、1个Rubisco活化酶(广泛存在于植物中调节Rubisco活性的酶)、1个二甲基萘醌甲基转移酶(一种参与甲基转移反应的辅酶)、1个推定的胞质型谷氨酰胺合成酶(参与高等植物氨同化过程的关键酶)、1个ATP合酶β亚基(在活性细胞中起着将其它能量合成为生物能量通货-ATP的能量转换作用)。【结论】揭示了红麻GA42表现出较强的耐旱性与上述6个差异表达蛋白质点明显上调有关。
Abstract： [Objective] The analysis of stress-responsiveness in kenaf is an important route to the discovery of genes conferring stress tolerance. Proteomic analysis provides a broad view of plant responses to stress at the level of proteins. [ Method ] A drought-resistant kenaf variety GA42 has been identified as materials by setting the normal water supply and water control comparison test at seeding stage, stress-induced proteome changes were analyzed by two-dimensional gel electrophoresis. [Result] In total, 9 up-regulated protein spots reproducibly presented differential expression patterns on 2-DE maps. Six spots were identified and confirmed by MALDI-TOF-TOF MS, including 2 ribulose-1, 5-bisphosphate carboxylase or large chain （a key enzyme in the first major step of carbon fixation）, 1 nabisco activase （a enzyme could regulate the activity of rubisco existing widely in plants）, 1 dimethylmenaquinone methyltransferase （a coenzyme participates in methyl transfer reactions）, 1 putative plastidic glutamine synthetase （a key enzyme participates in ammonia assimilation in higher plants）, and 1 ATP synthase subunit 13 （an important enzyme that provides energy for the cell to use through the synthesis of ATP, which is the most commonly used ＂energy currency＂ of the cellsfrom most organisms）. [Conclusion] This study preliminarily showed these six protein spots are up-regulated which are associated with GA42 resistance under drought stress.
出处
《中国农业科学》
CAS
CSCD
北大核心
2012年第17期3632-3638,共7页
Scientia Agricultura Sinica
基金
福建省发改委"五新"项目(SCZ10007)
福建省科技厅南方黄/红麻种质资源共享平台项目(2010N2002)
农业部黄/红麻东南科学观测实验站建设项目(农科教发2011-9)
关键词
红麻
干旱胁迫
差异蛋白
双向电泳
kenaf （Hibiscus cannabinus L.）
drought stress
differential protein
2-DE
