摘要
目的探讨石杉碱甲(huperzine A)改善急性低压低氧模型大鼠的空间记忆能力以及对模型大鼠脑组织氧化应激损伤的缓解作用。方法 48只大鼠随机分为4组:平原组(对照组)、平原给药组(0.1 mg.kg-1)、高海拔组(模拟6 000 m海拔)、高海拔给药组。比较各组大鼠Morris水迷宫学习与记忆测试成绩及大脑海马组织中谷胱甘肽、丙二醛含量和过氧化氢酶、超氧化物歧化酶、乳酸脱氢酶的活力。结果与高海拔组比较,高海拔给药组大鼠逃避潜伏期明显较短(P<0.05),60 s内穿越平台次数明显较多(P<0.05),目标象限滞留时间明显较长(P<0.05),海马组织中谷胱甘肽含量、超氧化物歧化酶、过氧化氢酶活力明显较高(P<0.05),丙二醛含量、乳酸脱氢酶活力明显较低(P<0.05),而高海拔给药组与平原对照组比较差异不显著。结论石杉碱甲对急性低压低氧导致的大鼠脑组织氧化应激损伤具有缓解作用,同时改善模型大鼠的空间记忆能力。
OBJECTIVE To investigate the effects of huperzine A in ameliorating acute hypobaric hypoxic-induced spatial memory deficits and in relieving oxidative stress injury in rat brain. METHODS A total of 48 rats were randomly divided into four groups, the champaign group （control group） , champaign ＋ huperzinc A group （0. 1 mg. kg-l ） , high altitude group （simulated 6 000 m plateau） and high altitude ＋ huperzine A group. The Morris water maze learning and memory test, the concentration of GSH, MDA as well as the activities of CAT, SOD and LDH in hippocampus were measured and compared. RESULTS Compared with those of high altitude group, escape latency of high altitude ＋ huperzine A group was significantly shorter （ P 〈 0. 05 ） , platform crossings within 60 s was significantly more （ P 〈 0. 05 ）, time spent in target was significantly longer （ P 〈 0. 05 ）, GSH content, SOD and CAT activity in hippocampal tissue were significantly higher （ P 〈 0. 05 ） , MDA and LDH activity in hippocampal tissue was significantly lower （ P 〈 0. 05 ） , and all these changes had no significantly difference as compared with the champaign group. CONCLUSION Huperzine A treatment has protective effects against acute hypobaric hypoxic-induced oxidative stress injury in rat brain, and ameliorates spatial memory deficits in rats.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2012年第17期1378-1381,共4页
Chinese Pharmaceutical Journal
基金
兰州军区医药卫生科研项目(CLZ11JB21)
新疆军区医院人才基金(联卫[2009]62号)
关键词
急性低压低氧
氧化应激
石杉碱甲
大鼠
海马
acute hypobaric hypoxia
oxidative stress
huperzine A
rats
hippocampus
